Effect of NK cells on GVHD in H-2 haploidentical bone marrow transplantation in mice |
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| Authors: | Mei Zhang Di Wu Hui Xu Pengcheng He Jing Li Jieying Xi Ruibo Cai Yalin Liu |
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| Affiliation: | Mei Zhang(Department of Hematology,the First Affiliated Hospital of Medical College of Xian Jiao Tong University,Xi'an 710061,China)；Di Wu(Department of Hematology,the First Affiliated Hospital of Medical College of Xian Jiao Tong University,Xi'an 710061,China)；Hui Xu(Department of Hematology,the First Affiliated Hospital of Medical College of Xian Jiao Tong University,Xi'an 710061,China)；Pengcheng He(Department of Hematology,the First Affiliated Hospital of Medical College of Xian Jiao Tong University,Xi'an 710061,China)；Jing Li(Department of Hematology,the First Affiliated Hospital of Medical College of Xian Jiao Tong University,Xi'an 710061,China)；Jieying Xi(Department of Hematology,the First Affiliated Hospital of Medical College of Xian Jiao Tong University,Xi'an 710061,China)；Ruibo Cai(Department of Hematology,the First Affiliated Hospital of Medical College of Xian Jiao Tong University,Xi'an 710061,China)；Yalin Liu(Department of Hematology,the First Affiliated Hospital of Medical College of Xian Jiao Tong University,Xi'an 710061,China)； |
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| Abstract: | Objective: To study the effect of natural killer (NK) cells on graft-versus-host disease (GVHD) after H-2 haploidentical bone marrow transplantation (BMT) in mice. Methods :Murine model of H-2 haploidentical BMT was established by using Balb/c (H-2d) mouse as recipient, and Balb/c(H-2d)×C57BL/6 (H-2b) (H-2d/b) mouse as donor. Lethally irradiated Balb/c (H-2d) mice were transplanted with the bone marrow cells from Balb/c(H-2d)×C57BL/6(H-2b) (H-2d/b) mice containing donor spleen cells and/or NK cells. GVHD and survival rates were studied by observation of clinical manifestations and pathological changes. Results:In the group of bone marrow +spleen cells, GVHD was induced in 90% mice; but in the group plus with low amount of NK cells,GVHD was induced in 20% mice; and in the group transplanted with high amount of NK cells, GVHD was induced only in 10% mice. Compared to the group transplanted only with BM plus spleen cells, the incidences of GVHD in the latter two groups decreased significantly (P＜0.01) and the survival rates at different periods of 15, 30, 45 and 60 days increased obviously (P＜0.01 ). Conclusion: In mouse H-2 haploidentical BMT, alloreactive NK cells can reduce the incidence of GVHD and increase the survival rate. |
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