益骨胶囊含药血清对大鼠成骨细胞IL-6 mRNA及其蛋白表达的影响

目的：从mRNA及蛋白水平探讨益骨胶囊含药血清对成骨细胞表达IL-6的影响。方法：分离、培养新生SD大鼠成骨细胞，传代后分为3组即含药血清组；空白血清组；DMEM组。采用RT-PCR法检测IL-6 mRNA相对表达量，用放射免疫法检测成骨细胞培养上清中的IL-6含量。结果：益骨胶囊含药血清组IL-6 mRNA相对表达量显著低于对照组（P<0.05）；益骨胶囊含药血清组IL-6蛋白表达量也低于对照组（P<0.05）。结论：益骨胶囊含药血清能下调成骨细胞IL-6 mRNA 表达；亦能在蛋白水平降低成骨细胞分泌骨吸收因子IL-6，这可能是益骨胶囊防治骨质疏松症的机制之一。

Effect of benefiting-bone Capsule on IL-6 mRNA and protein expression in rat osteoblasts in vitro

AIM: The effects of benefiting-bone Capsule (BBC) containing serum on IL-6 mRNA and protein expression in osteoblasts were studied.METHODS: (1) The neonate Sprague-dawley rat osteoblasts were cultured and divided into three groups: group Ⅰ (containing deactivating serum with BBC), group Ⅱ (containing deactivating serum without BBC) and group Ⅲ (DMEM medium group)； (2) RT-PCR was used to measure the relative IL-6 mRNA levels； (3) The radioimmunoassay method was used to examine IL-6 protein in the supernatant of the cultured osteoblasts. RESULTS: (1) The relative IL-6 mRNA levels was lower in group I than the control (P<0.05); (2) The IL-6 protein expression in osteoblasts was also lower in group I than the control (P<0.05).CONCLUSIONS: These results suggest BBC drug-containing serum can down-regulate the expression of IL-6 mRNA and protein in osteoblasts, which may be one of the mechanisms of BBC preventing and treating osteoporosis.