Measurement of immune complexes in insulin-treated diabetics |
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| Authors: | K S Jayarao W P Faulk J H Karam G M Grodsky P H Forsham |
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| Affiliation: | Metabolic Research Unit, University of California Medical Center, San Francisco, Calif. 94143, USA |
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| Abstract: | Insulin therapy produces anti-insulin antibodies the measurement of which by conventional radioimmunoassay depends on the presence of available combining sites to bind radiolabelled insulin. The availability of free antibody-combining sites depends on variables such as the molecular ratio of antibody to insulin, and the affinity of the various antibodies to the insulin antigen. An improved method for measuring insulin antibody in serum is described in which insulin-anti-insulin complexes are dissociated at acid pH and all liberated insulin is removed by charcoal adsorption prior to conventional radioimmunoassay. Increases in antibody titre after this acid dissociation charcoal-adsorption reflect the presence of circulating immune complexes in sera of diabetics. |
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| Keywords: | To whom reprint requests should be addressed. |
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