检测血清肌酸激酶同工酶MB质量和活性初探

目的 通过检测血清肌酸激酶同工酶(CK-MB)质量和活性,研究缺血性心脏疾病和非缺血性心脏疾病中的差异和应用.方法 应用酶免疫化学发生法与酶法分别测定心肌梗死、缺血性心脏疾病和非缺血性心脏疾病.结果 心肌梗死(AMI)16例,其CK-MB质量的灵敏度和特异度分别为93.75%和66.66%.肺部感染引发心脏缺血患者18例,其CK-MB质量的灵敏度和特异度分别为66.66%和57.89%.非心肌缺血患者20例,发现缺血性心脏疾病和非缺血性心脏疾病在阳性率、升高倍数等问题上CK-MB酶活性检测的假阳性率明显高于CK-MB质量检测.结论 CK-MB质量检测优于CK-MB活性检测,特别适用于AMI患者和伴有较明显肺部感染的心肌缺血患者的临床实验诊断.

Comparison and application of the quality and activity of serum CK-MB

Objective To study the difference and application between icshemic heart disease and non-ischemic heart disease through the quality and activity of serum CK-MB. Methods To determine AMI, ischemic heart disease and non-ischemic heart disease by applying immunosuppress and enzymology respectively. Results The sensitivity and specificity was 93.75% and 66. 66% in 16^# AMI. The sensitivity and specificity of 18 patients infected from the lung was 93.75% and 66. 66% respectively. While considering the false positive rate, high level ere, among 20 non-ischemic heart muscle patients, the false positive rate in activity of CK-MB was obviously higher than that in quality of CK-MB. Conclusion The quality of CK-MB is better than the activity of CK-MB in the performance, especially to AMI patients and ischemic heart muscle patients infected obviously. Secondly, the interference to the quality of CK-MB is lower than to the activity of CK-MB.