Real-time immunohistochemistry analysis of embedded tissue |
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| Authors: | Lars Gedda Hanna Björkelund Karl Andersson |
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| Affiliation: | 1. Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;2. Department of Radiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;1. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA;2. Division of Biochemical Diseases, Department of Pediatrics, British Columbia Children''s Hospital/University of British Columbia, Vancouver, BC, Canada;3. Department of Pathology and Laboratory Medicine, British Columbia Children''s Hospital/University of British Columbia, Vancouver, BC, Canada;4. Division of Pediatric Neurology, Department of Pediatrics, British Columbia Children''s Hospital/University of British Columbia, Vancouver, BC, Canada |
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| Abstract: | We present a novel analysis of membrane–protein expression in tissue sections based on semi-automatic real-time measurement using LigandTracer® technology. A commercial antiHER2 antibody developed for immunohistochemistry used in this setup was revealed to have sub-optimal interaction with tissue when analyzed as recommended for immunohistochemistry. We therefore think that real-time measurement of tissue, offering direct and quantitative membrane–protein interaction analysis, can lead to improved reproducibility and eliminate the subjective operator dependences that classical immunohistochemsitry suffers from. |
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