The effects of chemical and radioactive properties of Tl-201 on human erythrocyte glucose 6-phosphate dehydrogenase activity |
| |
Authors: | Ali Sahin Murat Senturk Mehmet Ciftci Erhan Varoglu Omer Irfan Kufrevioglu |
| |
Institution: | 1. Faculty of Medicine, Department of Nuclear Medicine, Ataturk University, Erzurum 25240, Turkey;2. Science Faculty, Department of Chemistry, Ataturk University, Erzurum 25240, Turkey;3. Science and Arts Faculty, Department of Chemistry, Agri Ibrahim Cecen University, 04100, Agri, Turkey |
| |
Abstract: | AimThe inhibitory effects of thallium-201 (201Tl) solution on human erythrocyte glucose 6-phosphate dehydrogenase (G6PD) activity were investigated.MethodsFor this purpose, erythrocyte G6PD was initially purified 835-fold at a yield of 41.7% using 2′,5′-Adenosine diphosphate sepharose 4B affinity gel chromatography. The purification was monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which showed a single band for the final enzyme preparation. The in vitro and in vivo effects of the 201Tl solution including Tl+, Fe+3 and Cu+2 metals and the in vitro effects of the radiation effect of the 201Tl solution and non-radioactive Tl+, Fe+3 and Cu+2 metals on human erythrocyte G6PD enzyme were studied. Enzyme activity was determined with the Beutler method at 340 nm using a spectrophotometer. All purification procedures were carried out at +4°C.Results201Tl solution and radiation exposure had inhibitory effects on the enzyme activity. IC50 value of 201Tl solution was 36.86 μl (Tl+]: 0.0036 μM, Cu+2]: 0.0116 μM, Fe+3]: 0.0132 μM), of human erythrocytes G6PD. Seven human patients were also used for in vivo studies of 201Tl solution. Furthermore, non-radioactive Tl+, Fe+3 and Cu+2 were found not to have influenced the enzyme in vitro.ConclusionHuman erythrocyte G6PD activity was inhibited by exposure for up to 10 minutes to 0.057 mCi/kg 201Tl solution. It was detected in in vitro and in vivo studies that the human erythrocyte G6PD enzyme is inhibited due to the radiation effect of 201Tl solution. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|