mini VITAL全自动血培养仪假阳性/假阴性结果分析

目的　评价miniVITAL全自动血培养仪在临床应用中的假阳性 /假阴性情况。方法　采用回顾性分析 ,对miniVITAL全自动血培养仪在检测 3834份临床标本中出现的假阳性 /假阴性率及导致假阴性结果的细菌种类与分布进行评估分析。结果　miniVITAL系统假阳性率和假阴性率分别为 0 .6 8%和 0 .70 % ,所有致假阴性菌种中 ,以真菌的比率最高 ,占 6 2 .0 7% ,其次为非发酵菌、草绿色链球菌、凝固酶阴性葡萄球菌、革兰阳性杆菌和厌氧菌 ,分别为 10 .34%、10 .34%、6 .90 %、6 .90 %和 3.4 5 %。结论　对miniVITAL系统血培养仪报告的阴性结果必须同时进行细菌和真菌的传代培养

Evaluation of false positive and false negative results for mini VITAL automated blood culture system

Objective To evaluation the false positive and false negative results for mini VITAL automated blood culture system in clinical application. Methods A total of 3 834 specimens were detected by the mini VITAL system. The false positive and false negative results were analyzed retrospectively. Results The false positive and false negative rates were 0.68% and 0.70%. Epiphyte was the main cause of the false negative results (62.07%), and the others were nofermenters (10.34%), Viridans streptococci (10.34%), coagulase negative Staphylococci (6.90%), Gram positive Corynebacterium (6.90%) and anaerobic bacteria (3.45%). Conclusions It is necessary to do subculture of bacterium and fungus for the negative results reported by mini VITAL system.