| RP-HPLC法测定咽炎糖浆中黄芩苷的含量 |
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| 引用本文： | 王晓明,王跃飞,潘桂湘. RP-HPLC法测定咽炎糖浆中黄芩苷的含量[J]. 齐鲁药事, 2007, 26(12): 718-720 |
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| 作者姓名： | 王晓明 王跃飞 潘桂湘 |
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| 作者单位： | 天津中医药大学中医药研究院,省部共建教育部方剂学重点实验室,天津,300193 |
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| 摘 要： | 目的建立咽炎糖浆中黄芩苷的含量测定方法。方法采用反相高效液相色谱法,色谱柱为依利特Hypersil ODS2 C18柱(4.6mm×250mm,5μm),甲醇-0.1%磷酸(46∶54)为流动相,流速1.0mL·min-1,紫外检测波长为280nm,柱温30℃,进样量10μL。结果咽炎糖浆中黄芩苷浓度在3.83~122.56μg.mL-1范围内呈良好的线性关系,平均回收率为100.74%,RSD为2.21%(n=6)。结论本法灵敏、简便、准确,重现性好,可用于咽炎糖浆中黄芩苷的含量测定。
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| 关 键 词： | 咽炎糖浆 黄芩苷 反相高效液相色谱法 |
| 文章编号： | 1672-7738（2007）12-0718-03 |
Determination of baicalin in Pharyngitis Syrup by RP-HPLC |
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| WANG Xiao-ming,WANG Yue-fei,PAN Gui-xiang. Determination of baicalin in Pharyngitis Syrup by RP-HPLC[J]. qilu pharmaceutical affairs, 2007, 26(12): 718-720 |
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| Authors: | WANG Xiao-ming WANG Yue-fei PAN Gui-xiang |
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| Abstract: | OBJECTIVE To determine the content of baicalin in Pharyngitis Syrup.METHODS The determination was carried out Hypersil ODS2 C18 column (4.6mm×250mm,5μm).The mixture of methanol-0.1% phosphoric acid (46∶54) was used as the mobile phase.The flow rate was 1.0mL·min-1.The UV detective wavelength was 280nm.The column temperature was 30℃,and the injection volume was 10μL.RESULTS The linear range of baicalin was 3.83~122.56μg·mL-1,the average recovery of this method was 100.74% and the RSD was 2.21%(n=6).CONCLUSION The method was sensitive,simple,accurate and reproducible,and suitable for the quality control of baicalin in Pharyngitis Syrup. |
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| Keywords: | Pharyngitis Syrup baicalin RP-HPLC |
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