MPTP处理的小鼠和6-OHDA损毁的大鼠帕金森病模型病理变化的比较

目的比较ＭＰＴＰ处理的小鼠与６－ＯＨＤＡ损毁大鼠ＰＤ模型的病理变化。方法应用免疫组化方法观察两种ＰＤ模型的中脑腹侧多巴胺能神经元，星形胶质细胞的变化。结果免疫组化方法结果表明：Ｃ５７ＢＬ小鼠在ＭＰＴＰ处理后，脑内酪氨酸羟化酶阳性神经元的数目从第４ｄ开始有所减少；黑质区域的ＧＦＡＰ免疫阳性星形胶质细胞数目从第１ｄ起即有增加。大鼠ＰＤ模型较早出现损毁侧ＴＨ免疫阳性神经元明显减少的现象，损毁２月后几乎完全消失；而在黑质区的ＧＦＡＰ免疫阳性星形胶质细胞数目增多现象出现较晚。结论两种动物模型从不同侧面反映了帕金森病的病理特征，６－ＯＨＤＡ损毁中脑ＤＡ能神经元制成的大鼠ＰＤ模型比ＭＰＴＰ处理的Ｃ５７ＢＬ小鼠ＰＤ模型更接近反映ＰＤ的病理变化。

Comparsion of the pathology changes between two kinds of PD animal models with M PTP-treated mice and 6-OHDA-lesioned rats

Objective To compare the pathology changes between two kinds of PD animal models with MPTP treated mice and 6 OHDA lesioned rats.Methods Thirty six C 57 BL male mice with 7 weeks old administrated subcutaneously by MPTP(30mg/kg) once every day were divided into different groups,according to their MPTP treated time.Twenty SD rats with unilateral 6 OHDA lesioned dopamine neurons in the midbrain,were used for PD models which were divided into two groups,according to postoperative survival time.The abnormal rotations could be induced by injection of apomorphine in all these rat models.Results The immunohistochemical staining showed that the loss of TH positive neurons did not appear until the 4th day for treating mice with MPTP,while a increase of GFAP positive astrocytes could be seen from lst day of injection.The immunohistochemical staining of rat brain sections showed that a significant loss of TH postive neurons in the lesion side could be observed from early stage,and completely disappeared from 2 months after lesion.The GFAP postive astrocytes in the same field,on the other hand,significantly increased rather late.Conclusion The pathology changes of both mouse and rat models could reflect PD ,s from different aspects,but rat PD model could reflect PD ,s better.