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血管紧张素Ⅱ对大鼠肾入球小动脉细胞内钙离子浓度的影响及Losartan的拮抗作用
引用本文:孙洞箫, 周盾, 邱振宇. 血管紧张素Ⅱ对大鼠肾入球小动脉细胞内钙离子浓度的影响及Losartan的拮抗作用[J]. 中国现代应用药学, 2010, 27(10): 857-860.
作者姓名:孙洞箫  周盾  邱振宇
作者单位:1.辽宁医学院附属第一医院肾内科,辽宁 锦州 121001
基金项目:辽宁省教育厅2008年资助项目(2008407)
摘    要:目的 探讨血管紧张素Ⅱ(AngⅡ)对肾小球入球小动脉细胞内钙离子浓度([Ca2+]i)的影响与高血压肾小动脉重建的关系及Losartan的治疗作用。方法 细胞培养:大鼠肾小球入球小动脉细胞随机分为4组。对照组:不加AngⅡ处理;AngⅡ组:加入终浓度为0.1 μmol·L-1的AngⅡ;Losartan组:加入终浓度为50 μmol·L-1的Losartan;AngⅡ+Losartan组:同时加入Losartan 50 μmol·L-1和AngⅡ0.1 μmol·L-1。负载后上机检测[Ca2+]i。结果 细胞培养显示AngⅡ引起了细胞收缩变化,表现为胞突变细变短,胞突回缩,胞体变圆,细胞长度与直径均明显缩小。Losartan处理组较AngⅡ组细胞形态变化减轻且Losartan对AngⅡ增高RASMCs内[Ca2+]i有明显抑制作用。结论 AngⅡ可引起肾小球入球小动脉细胞内[Ca2+]i的上升,导致细胞收缩,因此Ca2+超载可能是肾素-血管紧张素系统起作用的重要环节。Losartan可抑制细胞内Ca2+超载。

关 键 词:肾小球入球小动脉细胞  高血压  血管紧张素Ⅱ  Losartan  细胞内钙离子浓度

Effect of Angiotensin II on Intracellular Free Calcium Concentration in RASMCs and Antagonism of Losartan
SUN Dongxiao, ZHOU Dun, QIU Zhenyu. Effect of Angiotensin II on Intracellular Free Calcium Concentration in RASMCs and Antagonism of Losartan[J]. Chinese Journal of Modern Applied Pharmacy, 2010, 27(10): 857-860.
Authors:SUN Dongxiao  ZHOU Dun  QIU Zhenyu
Affiliation:1.Department of Nephorology, the First Hospital Affiliated Liaoning Medical College, Jinzhou 121001, China
Abstract:OBJECTIVE To explore the effects of angiotensin(Ang Ⅱ) on intracellular free calcium concentration([Ca^2+]i) in RASMCs and antagonism of Losartan.METHODS RASMCs were isolated and divided into four groups: control group,Ang Ⅱ group,Losartan group and Ang Ⅱ +Losartan group.Confocal microscope was used with Fluo-3/AM as an indicator to detect the changes of [Ca^2+]i.RESULTS Compared with control group,intracellular fluorescent intensity of RASMCs had no change in Losartan group,and was significantly higher in Ang Ⅱ group(P〈0.05).Value in Ang Ⅱ +Losartan group was significantly lower than that in Ang Ⅱ group(P〈0.05).CONCLUSION Ang Ⅱ may induce intracellular calcium overload in RASMCs,which can be significantly reduced by Losartan.
Keywords:RASMCs  hypertension  angiotensin Ⅱ  Losartan  intracellular free calcium concentration
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