| IL-10基因修饰对骨髓间充质干细胞体外免疫效应的影响 |
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| 引用本文： | 方针强,戴 玮,贾维胜,何 凡,沈崇星,刘永亮,周锋,叶 钢. IL-10基因修饰对骨髓间充质干细胞体外免疫效应的影响[J]. 医学教育探索, 2013, 0(11) |
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| 作者姓名： | 方针强 戴 玮 贾维胜 何 凡 沈崇星 刘永亮 周锋 叶 钢 |
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| 作者单位： | 第三军医大学新桥医院泌尿外科,,,,,,, |
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| 基金项目： | 重庆市科委自然科学基金计划资助项目(CSTC, 2009BB5154) |
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| 摘 要： | 目的：研究IL-10基因修饰的骨髓间充质干细胞（BM-MSCs）体外免疫效应。方法:RT-PCR克隆大鼠IL-10基因并构建逆转录病毒pLNCX-IL-10，转染大鼠BM-MSCs；流式细胞术检测IL-10基因修饰的BM-MSCs（BM-MSCsIL-10）体外对T淋巴细胞亚群的影响，CCK-8法检测BM-MSCsIL-10及其培养上清液对体外混合淋巴细胞增殖的影响。结果:BM-MSCsIL-10能明显减少CD4 T，降低CD4 /CD8 比值，比BM-MSCs作用明显（P<0.05）；BM-MSCsIL-10及其培养上清液比BM-MSCs更能明显抑制T淋巴细胞增殖反应（P<0.05）。结论:IL-10基因修饰能增强BM-MSCs的体外免疫抑制作用。
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| 关 键 词： | 骨髓间充质干细胞；白细胞介素-10；免疫抑制 |
Influence of IL-10 modification on in vitro immunosuppressive effect of bone marrow mesenchymal stem cells |
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| fangzhenqiang,and. Influence of IL-10 modification on in vitro immunosuppressive effect of bone marrow mesenchymal stem cells[J]. Researches in Medical Education, 2013, 0(11) |
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| Authors: | fangzhenqiang and |
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| Abstract: | Objective: To study the in vitro immunosuppressive effect of IL-10 modified bone marrow mesenchymal stem cells(BM-MSCsIL-10). Method: IL-10 was cloned by RT-PCR and was used to construct pLNCX-IL-10. The constructed retrovirus was transfected into rat BM-MSCs; T lymphocyte subsets in vitro influenced by BM-MSCsIL-10 were detected by flow cytometry. BM-MSCsIL-10 or its culture supernatant on in vitro mixed heterologous T lymphocyte proliferation were detected by CCK-8. Results: BM-MSCsIL-10 significantly reduced CD4 T and lowered the ratio of CD4 /CD8 more than BM-MSCs (P <0.05); BM-MSCsIL-10 and BM-MSCsIL-10 or its culture supernatant significantly inhibited T lymphocyte proliferation more than BM-MSCs (P <0.05). Conclusion: IL-10 modification can enhance the in vitro immunosuppressive effect of BM-MSCs. |
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| Keywords: | bone marrow mesenchymal stem cells IL-10 immunosuppression |
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