| 丹参酮对肥大心肌细胞中电生理特征和钙调神经磷酸酶活性的作用 |
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| 引用本文： | 王照华,李永胜,杨乐,梁黔生.丹参酮对肥大心肌细胞中电生理特征和钙调神经磷酸酶活性的作用[J].现代中西医结合杂志,2011,20(32):4060-4063. |
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| 作者姓名： | 王照华 李永胜 杨乐 梁黔生 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院,湖北,武汉,430030 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的观察丹参酮对肥大心肌细胞中电生理特征和钙调神经磷酸酶(CaN)活性的作用。方法通过膜片钳、胞内钙测定和分子生物学技术,观察丹参酮对"一肾一夹"手术导致的肥厚心肌细胞膜上动作电位时间、L型钙通道电流(ICa,L)、胞内钙离子浓度以及CaN活性的影响。结果丹参酮可以显著缩短肥厚心肌细胞中存在的动作电位时间延长(P<0.001)、降低膜电容和ICa,L峰值幅度(P均<0.001),但不影响ICa,L密度,并能够显著减少胞内钙离子浓度。丹参酮能显著抑制肥厚心肌中CaN的表达(P<0.05)。结论丹参酮可以有效地减少肥大细胞中的Ca2+浓度并抑制L-型钙通道,并使肥大心肌细胞中的CaN活性降低,从而改善肥大心肌细胞中存在的电生理异常和减轻心肌肥厚的发生。
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| 关 键 词： | 丹参酮 心肌肥厚 电生理异常 钙调神经磷酸酶 |
Action of tanshinone on electrophysiological abnormality and calcineurin activity in hypertrophy cardiomyocyte |
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| Wang Zhaohua,Li Yongsheng,Yang Le,Liang Qiansheng.Action of tanshinone on electrophysiological abnormality and calcineurin activity in hypertrophy cardiomyocyte[J].Modern Journal of Integrated Chinese Traditional and Western Medicine,2011,20(32):4060-4063. |
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| Authors: | Wang Zhaohua Li Yongsheng Yang Le Liang Qiansheng |
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| Institution: | Wang Zhaohua,Li Yongsheng,Yang Le,Liang Qiansheng(Tongji Hospital Affiliated to Tongji Medical College,Huangzhong University of Science and Technology,Wuhan 430030,Hubei,China) |
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| Abstract: | Objective It is to observe the action of tanshinone on electrophysiological abnormality and calcineurin(CaN) activity in hypertrophy cardiomyocyte.Methods The influence of tanshinone on action potential duration,L-type calcium channels current(ICa,L),intra-cellular calcium ion concentration and CaN activity in cellular membrane of hypertrophic myocardium induced by the surgery of one kidney one clamp was observed through patch clamp,intra-cellular calcium mensuration and molecular biological technique.Resul... |
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| Keywords: | tanshinone myocardial hypertrophy electrophysiological abnormality calcineurin |
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