Detections of HFRS Virus Antigen and Antibody by a McAb ELISA Indirect Sandwich Method

An ELISA indirect sandwich method using monoclonal antibodies(McAb)against hem-orrhagic fever with renal syndrome(HFRS)virus has been developed fordetections of HFRS virus antigen and specific antibody.Dynamic changes of HFRS virusantigens in the cells infected and their culture supernatants were observed by indirectfluorescence-antibody technique(IFAT)and ELISA separately.It was shown that HFRSvirus antigens in the cells and in the supernatants peaked separately 8 days and 14 daysafter infection.A hundred and seventy-nine brain and lung specimens of suckling miceinfected were examined by ELISA and IFAT.The positive rates were 72.1% and 68.2%,respectively.Furthermore,the IgM antibodies of 121 sera from HFRS patients wereexamined by ELISA and IFAT.The positive rates were 90.1% and 83.5% respectively.The IgG antibodies of 178 sera from HFRS patients were also examined by the twomethods,and both of the positive rates were 89.3%.The antibody titer measured byELISA was higher than the titer by IFAT.The above results suggest that the ELISA is aspecific and sensitive method and can be used for early diagnosis,epidemiologicalsurveillance and etiological study of HFRS.

Detections of HFRS Virus Antigen and Antibody by a McAb ELISA Indirect Sandwich Method

An ELISA indirect sandwich method using monoclonal antibodies(McAb) against hem-orrhagic fever with renal syndrome(HFRS)virus has been developed for detections of HFRS virus antigen and specific antibody.Dynamic changes of HFRS virus antigens in the cells infected and their culture supernatants were observed by indirect fluorescence-antibody technique(IFAT)and ELISA separately.It was shown that HFRS virus antigens in the cells and in the supernatants peaked separately 8 days and 14 days after infection.A hundred and seventy-nine brain and lung specimens of suckling mice infected were examined by ELISA and IFAT.The positive rates were 72.1% and 68.2%, respectively.Furthermore,the IgM antibodies of 121 sera from HFRS patients were examined by ELISA and IFAT.The positive rates were 90.1% and 83.5% respectively. The IgG antibodies of 178 sera from HFRS patients were also examined by the two methods,and both of the positive rates were 89.3%.The antibody titer measured by ELISA was higher than the titer by IFAT.The above results suggest that the ELISA is a specific and sensitive method and can be used for early diagnosis,epidemiological surveillance and etiological study of HFRS.