抗TNFR1中和抗体对急性肝衰竭小鼠肝细胞的保护作用

目的探讨抗TNFR1中和抗体对急性肝衰竭小鼠肝细胞的作用。方法联合应用D-氨基半乳糖和肿瘤坏死因子-α造模，酶联免疫吸附试验测定血清ALT、AST浓度。HE染色检查肝脏组织病理变化、脱氧核糖核苷酸转移酶介导的缺口末端标记（TUNEL）技术检测肝细胞的凋亡程度。结果模型组各时间点（4、8、12h）均可见明显的肝细胞凋亡，治疗组各时间点亦可见少量凋亡细胞，但与模型组比较各时间点均明显减少（P〈0．01），其血清ALT、AST浓度较模型组也有下降。结论抗TNFR1中和抗体对TNF—α能诱导肝细胞损伤具有保护作用肝。

The protection of anti-TNFR1 neutralizing antibody toward the hepatocytes of the acute hepatic failure mice

Objective To study the roles of anti-TNFR1 neutralizing antibody for hepatocytes of the acute hepatic failure mice. Methods Seventy-two male mice were divided into three groups randomly. The model group were induced of TNF-α and D-Ga1N. The treatment group were administered anti-TNFR1-Ab,TNF-α and D-Ga1N. The histopatho- logical change was observed by HE and TUNEL. Results Profuse apoptosis and necrosis emerged in hepatocytes when tested by TUNEL and HE method. However, in the treatment group, the liver injury was remarkably ameliorated based on the serum transaminase levels and histological change. Conclusion Anti-TNFR1-Ab treatment provides the basis for a novel therapeutic modality in which an unfavorable apoptotic process can be inhibited in acute hepatic failure mice.