胆红素结石病兔模型的粘液组织化学研究

作者通过胆红素结石病兔模型,用粮液组织化学方法对胆红素结石成石过程中硫酸粘多糖的来源、性质、含量与分布进行了动态对比观察,发现胆囊及胆管粘膜下的腺体增生和分泌亢进是感染条件下色素石成石机制中的一种基本病理改变,且与人类胆红素结石病人肝胆系统病理改变极为相似。

MUCUS HISTOCHEMICAL STUDY OF BILIRUBIN CHOLANGIOLITHIASIS IN RABBIT MODEL

Sulfated mucopolysaccharides have animportant role in pigment gallstone forma-tion. In this experiment, the animalmodel of bilirubin cholangiolithiasis wasmade with Japanese hybrid big-ear whiterabbits. The source, nature quantity anddistribution of sulfated mucopolysaccharidesin the cause of bilirubin cholangiolithiasiswere observed by means of mucous histo-chemical study. There were three characteristic patho-logic changes observed in this experiment:1. In normal condition, the sulfated muco-polysaccharides were secreted by epithiliumof biliary tracts and the quantity was mini-mum. When bacterial infection was presentin the biliary tracts, they were secretedmainly by the proliferative glands in sub-mucosa of the bile duct; 2. In 26 rabbits wherethe bilirubin cholangiolithiasis developed,there were many proliferative glands insubmucosa of the bile duct. Most of theglands produced sulfonated acid mucin. In 5rabbits where the gallstones did not developin the stone growing stage, the proliferativeglands were not present in the bile duct. Itwas suggested that there was a close rela-tionship between the proliferative glandsand the formation of bilirubin cholangio-liths; and 3. The glands in submucosa ofthe biliary tract provided the refuge wherethe bacteria could not be cleaned outeasily and so it was difficult to control theinfection of the biliary tract.