| 一种胶质瘤相关的酸性成纤维细胞生长因子mRNA同源物 |
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| 引用本文： | 邓艳春,药立波,王吉村,刘新平,聂晓燕,季少平,李福洋. 一种胶质瘤相关的酸性成纤维细胞生长因子mRNA同源物[J]. 医学争鸣, 2001, 22(6): 504-507 |
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| 作者姓名： | 邓艳春 药立波 王吉村 刘新平 聂晓燕 季少平 李福洋 |
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| 作者单位： | 第四军医大学基础部生物化学与分子生物学教研室, |
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| 基金项目： | 国家杰出青年基金!资助项目 ( 3 982 5 113） |
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| 摘 要： | 目的 克隆胶质瘤相关基因。方法 取胶质瘤患者新鲜肿瘤标本及肿瘤附近正常脑组织提取mRNA，反转录成cDNA，采用基于PCR的消减杂交法获得差异的cDNA，并经多聚酶链反应扩增后克隆人T载体测序，用打点杂交法验证差异克隆。结果 获得一胶质瘤相关的酸性成纤维生长因子基因同源物。结论 该酸性成纤维生长因子基因同源物是酸性成纤维生长因子mRNA的不同拼接产物，可能参与胶质瘤的形成。
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| 关 键 词： | 神经胶质瘤 杂交 肿瘤相关基因 酸性成纤维细胞生长因子 mRNA |
| 文章编号： | 1000-2790(2001)06-0504-04 |
| 修稿时间： | 2000-01-19 |
Glioma related homolog of acid fibroblast growth factor gene |
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| DENG Yan Chun,YAO Li Bo,WANG Ji Cun,LIU Xin Ping,NIE Xiao Yan,JI Sao Ping,LI Fu Yang. Glioma related homolog of acid fibroblast growth factor gene[J]. Negative, 2001, 22(6): 504-507 |
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| Authors: | DENG Yan Chun YAO Li Bo WANG Ji Cun LIU Xin Ping NIE Xiao Yan JI Sao Ping LI Fu Yang |
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| Affiliation: | DENG Yan Chun,YAO Li Bo,WANG Ji Cun,LIU Xin Ping,NIE Xiao Yan,JI Sao Ping,LI Fu Yang Department of Biochemistry and Molecular Biology,Faculty of Preclinical Medicine,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM To search for glioma related genes. METHODS Glioma sample and normal brain tissue of a patient were got under operation. mRNAs were extracted and reverse transcribed into cDNA. Subtractive hybridization was done to get the glioma specific cDNA which was amplified by polymerase chain reaction (PCR). The PCR products were cloned into vectors for sequencing. Dot hybridization was used to testify the different clone. RESULTS A homolog of acid fibroblast growth factor (aFGF)mRNA was found in tumor tissue but not in normal brain tissue. CONCLUSION The new gene is the different splicing product of aFGF and might be involved in glioma genesis. |
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| Keywords: | nerve glioma hybridization tumor related gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
