| 分光光度-白细胞黏附抑制法测定免疫核糖核酸的活性 |
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| 引用本文： | 乐嘉静,李湛军,刘丽华,徐康森.分光光度-白细胞黏附抑制法测定免疫核糖核酸的活性[J].中国生化药物杂志,2002,23(1):19-21. |
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| 作者姓名： | 乐嘉静 李湛军 刘丽华 徐康森 |
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| 作者单位： | 中国药品生物制品检定所,北京,100050 |
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| 摘 要： | 目的建立免疫核糖核酸活性测定方法。方法细胞浓度在 2× 10 6～ 2× 10 7,波长 415nm处吸收值与细胞浓度呈线性关系 ,可用吸收值测出细胞浓度 ,求出免疫核糖核酸的黏附抑制率。结果此法与细胞计数 白细胞黏附抑制 (LAI)实验有良好的相关性 ,r=0 .96 ,P <0 .0 1,重复性实验结果为 :板内RSD值为 6 .3% ,板间RSD值为 5 .4% ,不同实验次数RSD值为 9.4% ,灵敏度 0 .4mg/ml并能反应出免疫核糖核酸的抗原特异性。结论此法特异、简便并具有良好的重复性 ,可以作为免疫核糖核酸的活性测定方法
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| 关 键 词： | 免疫核糖核酸 分光光度法 白细胞黏附抑制实验 活性 |
| 文章编号： | 1005-1678(2002)01-0019-03 |
| 修稿时间： | 2001年3月8日 |
Determination of the biological activity of immune RNA by spectrophotometry-LAI test |
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| LE Jia jing,LI Zhuan jun,LIU Li hua,XU Kang sen.Determination of the biological activity of immune RNA by spectrophotometry-LAI test[J].Chinese Journal of Biochemical Pharmaceutics,2002,23(1):19-21. |
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| Authors: | LE Jia jing LI Zhuan jun LIU Li hua XU Kang sen |
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| Abstract: | PurposeThe aim is to develop a method for determining the biological activity of immune RNA.MethodsUtilization OD value with a linear range of cell consistency 2×10 6～2×10 7 was plotted.Using the OD value reflected cell consistency and determined adhesion inhibition rate of Immune RNA.ResultsThe method hada good correlativity with counted cell LAI, r =0.96,and P <0.01,tha cofficients of variataion of reproducibiilty of intra , inter and different test assays were 6.3%～5.0% and 9.4% respectively.ConclusionThe method was superfine, simple and highly reproducible. It can be the method for determining the biological activity of immune RNA. |
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| Keywords: | immune RNA spectrophotometry leukoeyte adherence inhibition activity |
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