生物型纳米根管封闭剂的生物学性能研究

目的：对磷酸钙骨水泥为原料制备的生物型纳米根管封闭剂进行体外生物学性能研究,为其临床应用提供依据。方法：致敏性实验：依照GB/T16886.10-2005和GB/T16886.12-2005技术标准测定;细胞毒性实验：依照GB/T16886.5-2003技术标准,采用琼脂扩散法测定;鼠伤寒沙门氏杆菌回复突变试验（Ames试验）：依照YY/T0127.10-2001技术标准测定。结果：生物型纳米根管封闭剂无致敏性,不导致染色体畸变,具有轻度细胞毒性。结论：生物型纳米根管封闭剂具有良好的生物学性能。

A study on the biological properties of a new bio-type root canal filling sealer in vitro

Objective:To assess the biological properties of a new root canal filling sealer produced by calcium acid phosphate bone cement in vitro, and provide the evidence for clinical trial. Method:This study is composed of three parts. Allergization test was conducted based on the standard technique of GB/T16886. 10-2005 and GB/T16886.12-2005;Agar diffusion method was used for Cytotoxicity test according to the GB/T16886.5-2003. Salmonella typhimurium reverse mutation test (Ames test) was performed based on items of technique standard of YY/T0127.10-2001. Result:The bio-type root canal filling sealer has no feature of allergization with only slight cytotoxicity, and no chromosomal aberration was found in the Ames testcytotoxicity. Conclusion:The bio-type root canal filling sealer has excellent biological properties, and can be used in clinical trial safely.