| RP-HPLC法同时测定升麻中3种有机酸的含量 |
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| 引用本文： | 司丹丹,李清,陈晓辉,康莉,冯毅,毕开顺. RP-HPLC法同时测定升麻中3种有机酸的含量[J]. 沈阳药科大学学报, 2007, 24(12): 754-757 |
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| 作者姓名： | 司丹丹 李清 陈晓辉 康莉 冯毅 毕开顺 |
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| 作者单位： | 1. 沈阳药科大学,药学院,辽宁,沈阳,110016
2. 河北省医疗器械与药品包装材料检验所,河北,石家庄,050061 |
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| 摘 要： | 目的建立同时测定升麻中咖啡酸、阿魏酸和异阿魏酸含量的方法。方法采用RP-HPLC法,C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水-磷酸(13.00∶87.00∶0.05),检测波长为320 nm。结果咖啡酸、阿魏酸和异阿魏酸的质量浓度分别在2.66~26.60 mg.L-1(r=0.999 5)、6~60 mg.L-1(r=0.999 3)和20.6~206.0 mg.L-1(r=0.999 3)内与峰面积呈良好的线性关系(n=6),方法回收率(n=9)分别为99.8%、98.2%和101.0%。结论RP-HPLC法可用于升麻中3种有机酸含量的同时测定。
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| 关 键 词： | 升麻 咖啡酸 阿魏酸 异阿魏酸 反相高效液相色谱法 |
| 文章编号： | 1006-2858（2007）12-0754-04 |
| 收稿时间： | 2006-12-14 |
| 修稿时间： | 2006-12-14 |
RP-HPLC determination of caffeic, ferulic acid, and isoferulic acid in Rhizoma Cimicifugae |
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| SI Dan-dan,LI Qing,CHEN Xiao-hui,KANG Li,FENG Yi,BI Kai-shun. RP-HPLC determination of caffeic, ferulic acid, and isoferulic acid in Rhizoma Cimicifugae[J]. Journal of Shenyang Pharmaceutical University, 2007, 24(12): 754-757 |
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| Authors: | SI Dan-dan LI Qing CHEN Xiao-hui KANG Li FENG Yi BI Kai-shun |
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| Abstract: | Objective To establish a method for the simultaneous determination of caffeicacid,ferulic acid and isoferulic acid in Rhizoma Cimicifugae.Methods The reversed phase HPLC system consisted of a C18 column(250 mm×4.6 mm,5 μm) and a mixture of acetonitrile-water-phosphoric acid(13:87:0.05)as the mobile phase.The absorbance was monitored at the wavelength of 320nm.Results The linear response range was 2.66-26.60 mg·L-1(r=0.999 5),6-60 mg·L-1(r=0.999 3) and 20.6-206.0 mg·L-1(r=0.999 3),respectively(n=6).The recoveries(n=9) of caffeicacid,ferulic acid and isoferulic acid were 99.8%,98.2% and 101.0%,respectively.Conclusions The method is simple and accuracy with good reproducibility and suitable for the measure of the concentrations of caffeicacid,ferulic acid and isoferulic acid in Rhizoma Cimicifugae. |
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| Keywords: | Rhizoma Cimicifugaeacid caffeic ferulic acid isoferulic acid RP-HPLC |
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