GDNF及HSV—GDNF对坐骨神经损伤大鼠脊髓运动神经元Bcl—2表达的影响

目的：观察胶质细胞源性神经营养因子（GDNF）及单纯疱疹病毒（HSV）载体介导的GDNF（HSV－GDNF）对坐骨神经损伤大鼠脊髓运动神经元了Bcl-2表达的影响。方法：分别取坐骨神经损伤后4d、7d和14d大鼠（）分成对照组、GDNF组和HSV－GDNF组）的腰段脊髓）L4－6），行石蜡包埋，切片、；用抗Bcl-2抗血清进行免疫组织化学染色，观察Bcl-2免疫反应（Bcl-2－IR）神经元数目，并在图像分析仪Bcl-2－IR阳性神经元作光密度的色谱分析。结果：1．坐骨神经损伤后4d、7d，GDNF组和HSV－GDNF组损伤侧脊髓运动神经Bcl-2－IR阳性神经元的数量和平均光密度均明显高于对照组损伤测，2．坐骨神经损伤后14d时，对照组、GDNF组和HSV－GDNF组损伤侧脊髓运动神经元对Bcl-2的表达已无明显差别。结论GDNF与HSV－GDNF能够增强坐骨神经扣内务 大鼠脊髓运动神经元Bcl-2的表达，减少神经元的退化死亡。

EFFECTS OF BOTH GDNF AND HSV-GDNF ON Bcl-2 EXPRESSION IN SPINAL CORD MOTOR NEURONS AFTER SCIATIC NERVE INJURY IN RATS

Objective To observe the effects of GDNF and HSV GDNF on Bcl 2 expression in spinal cord motor neurons after sciatic nerve injury in rats. Methods Lumbar of spinal cord was immunohistochemically stained by using the antiserum of Bcl 2.The number and mean optical densities of Bcl 2 immunoreactive(Bcl 2 IR)positive neurons were counted and observed. Results The number and mean optical densities of Bcl 2 IR positive neurons were significantly increased in GDNF and HSV GDNF 4 days and 7 days after sciatic nerve injury.But differences of the number and the mean optical densities of Bcl 2 IR positive neurons were not obvious in three groups 14 days after sciatic nerve injury. Conclusion Local application of GDNF and HSV GDNF to transected sciatic nerve can prevent the massive neuron apoptosis or programmed cell death induced by nerve injury.