针刺对局灶性脑缺血大鼠脑细胞凋亡的影响

以热凝法阻断大鼠一侧大脑中动脉 (MCAo)造成局灶性脑缺血为实验模型 ,用原位末端标记 (TUNEL方法 )技术观察缺血区脑细胞凋亡的动态变化及针刺的干预作用。结果表明 ,MCAo后 1hr脑缺血区已出现细胞凋亡 ,2 4hr达到高峰。凋亡细胞主要局限于梗塞灶中心坏死区域的周边“半影区”。“半影区”的细胞凋亡可能对梗塞灶的扩大起着重要作用。“醒脑开窍”针法能使缺血区脑细胞凋亡明显减少 ,从而减小梗塞面积 ,起到脑保护作用

Effect of Acupuncture on Cerebral Apotosis in Rats with Local Cerebral Ischemia

Objective: To observe changes of apoptosis in the cerebral ischemic area and the intervention effect of acupuncture in the rat. Methods: 160 Wistar rats were randomly divided into pseudo operation group (n=10), model group (n=50), “Xingnao" needling (XN) group (n=50) and routine acupuncture (RA) group (n=50), the last 3 groups were further divided into 1 hr, 3 hr, 6 hr, 24 hr and 48 hr subgroup, with 10 cases in each subgroup. In XN group, “Neiguan" (PC 6) and “Shuigou" (GV 26) were punctured and stimulated with “Xingnao" needling, and in RA group, “Quchi" (LI 11) and “Zusanli” (ST 36) were punctured and stimulated with routine needling method. Cerebral ischemia model was produced by thermocagulation occlusion of unilateral middle cerebral artery. Apoptosis staining was performed with TUNEL technique. Results: Along with the increase of cerebral ischemic duration, the count of apoptotic neurons mainly in the “penumbra area" around the central necrosis region raised gradually and peaked at the 24 th hr after ischemia. After acupuncture treatment, the counts of apoptotic cells in cerebral cortex, striate body and pre optic hypothalamic area in all the subgroups of XN and most subgroups of RA group decreased significantly in comparison with model group (P< 0.05, 0.01). The effect of XN was superior to that of RA. Conclusion: “Xingnao Kaiqiao" needling method can significantly relieve apoptosis and reduce cerebral infarction area.