| 三种方法检测乙肝表面抗原的结果分析 |
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| 引用本文： | 王厚照,马芳芳,陈云,谢则金.三种方法检测乙肝表面抗原的结果分析[J].临床军医杂志,2010,38(5). |
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| 作者姓名： | 王厚照 马芳芳 陈云 谢则金 |
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| 作者单位： | 解放军第174医院,检验科,福建,厦门,361003 |
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| 摘 要： | 目的探讨三种方法对乙肝表面抗原的定性和定量检测的结果并进行对比分析。方法采用临床上常用的胶体金方法、酶联免疫吸附实验法(ELISA法)、化学发光微粒子免疫分析法(CMIA法)对400例标本进行乙肝表面抗原检测,分别计算ELISA法、CMIA法、胶体金法检测结果的阳性率、灵敏度和特异度。结果 CMIA方法检测乙肝表面抗原的阳性率、灵敏度和特异度高于ELISA法和胶体金法。结论 CMIA法检测乙肝病毒标志物特异性强、灵敏度高,具有很高的临床应用价值。
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| 关 键 词： | 乙肝表面抗原 酶联免疫吸附测定 化学发光 |
Analysis on outcomes of three detection methods to hepatitis B surface antigen |
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| Wang Hou-zhao,Ma Fang-fang,Chen Yun,Xie Ze-jin.Analysis on outcomes of three detection methods to hepatitis B surface antigen[J].Clinical Journal of Medical Officer,2010,38(5). |
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| Authors: | Wang Hou-zhao Ma Fang-fang Chen Yun Xie Ze-jin |
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| Abstract: | Objective To compare the results from qualitative and quantitative detection of hepatitis B surface antigen(HbsAg).Methods A total of 400 serum specimens were qualitatively and quantitatively measured by immunoassay of colloidal gold,enzyme-linked immunosorbent assay(ELISA) and chemiluminescence microparticle immunoassay(CMIA).Results The positve rate,sensitivity and specificity of CMIA were higher than that of ELISA or immunoassay of colloidal gold.Conclusion CMIA is clinically suitable for the quantitative detection of hepatitis B surface antigen. |
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| Keywords: | hepatitis B surface antigen enzyme-linked immunosorbent assay chemiluminescence |
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