四种恶性血液病细胞系的Id4基因启动子甲基化

本研究探讨4种白血病和淋巴瘤细胞系以及非肿瘤细胞系和正常人骨髓Id4基因启动子甲基化状态及其差异。采用甲基化特异性聚合酶链反应（MS-PCR）对白血病细胞系K562和HL-60、淋巴瘤细胞系Ramous和CA46以及良性细胞系Hek937和正常人骨髓细胞进行Id4基因甲基化状态检测。结果表明：Id4基因在正常人骨髓细胞和Hek937细胞系中呈完全非甲基化状态，在4个血液肿瘤细胞系K562、HL-60、Ramous和CA46中均呈甲基化状态。结论：所检测的4种血液肿瘤细胞系中，Id4基因的甲基化状态与正常人骨髓细胞和非肿瘤细胞系完全不同，Id4基因甲基化模式的改变与造血细胞恶变的发生密切相关。

Methylation of Id4 Gene Promoter in 4 Malignant Hematopoietic Cell Lines

This study was purposed to investigate the status of Id4 gene promoter methylation in malignant hematologic cell lines.MS-PCR methods were used to detect the status of Id4 gene methylation in K562,HL-60,Ramous and CA46.Meanwhile bone marrow cells in healthy individuals and Hek937,a benign renal cell line,were involved as control.The results showed that Id4 gene kept unmethylated in bone marrow cells from healthy individuals and Hek937 cells,while Id4 gene was methylated in all of K562,HL-60,Ramous and CA46 cell lines.It is concluded that different from healthy bone marrow cells and non-malignant cell line Hek937,Id4 gene was methylated in leukemia and lymphoma cell lines including K562,HL-60,Ramous and CA46.The change of Id4 gene methylation is thought to be associated with occurrence of hematologic malignancies.