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Identification of type A and B isolates of Epstein-Barr virus by polymerase chain reaction
Authors:Wolfgang Jilg  Emilia Sieger  Peter Alliger and Hans Wolf
Institution:

Max von Pettenkofer-lnstitute for Hygiene and Medical Microbiology, University of Munich, Munich, F.R.G.

Abstract:A method is described for the identification of type A and type B isolates of Epstein-Barr virus (EBV) by means of the polymerase chain reaction. The use of three pairs of primers specific for genomic sequences coding for the two forms of EBV nuclear antigen (EBNA), 2A and 2B, and for a DNA sequence from the BamZ/BamR region allows the reliable and rapid detection of type A and B viruses in as little as 1000 EBV positive cells.
Keywords:(EBV)  Epstein-Barr virus  type A and B  Epstein-Barr virus nuclear antigen (EBNA) 2A and 2B  Polymerase chain reaction
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