半夏不同种质资源AFLP指纹系谱分析及其应用

目的:获得更多半夏DNA指纹信息,验证AFLP与其他方法是否一致,为半夏及其他中药材道地性研究、种质资源鉴定、亲缘关系研究、品种选育和引种栽培提供分子水平生物学依据。方法:选择全国10个主要产地的野生或栽培半夏为材料,采用扩增酶切片段多态性AFLP方法研究,用NTSYSpc 2.1软件包利用UPGMA法进行聚类分析,构建遗传系统树。结果:筛选12对引物,共扩增出1 673条带。进行聚类分析,结果与利用总生物碱含量差异结果相吻合。结论:本研究所获得的特征带和缺失带体现了AFLP的高特异分辨率在中药半夏品种鉴定上的潜力,绘制的系统进化树不同种源之间遗传关系的远近与其总生物碱含量差异趋势一致。

AFLP fingerprinting of Pinellia ternata and its application

Objective: To obtain more information on DNA fingerprintings and to confirm the genetic relationships of ten wide populations of Pinellia tornata.Method: Collect wide-type or cultivated P.tornata from ten major production areas nation-wide as material.Twelve combinations of EcoR I and Mse I primers were used to asses divergence among ten populations.The data were analyzed using unweighted pair-group method,based on arithmetic averages(UPGMA) bootstrap analysis.Cluster analyses were performed by using NTSYSpc software version 2.1.The alkaloid was extracted from P.ternate with chlorolform and determined in UV absorption spectroscop.Result: 1 673 bands were applied with 12 combinations.These results of cluster were congruent with differences in total alkloids.Conclusion: The results of the study showed a potential application of AFLP fingerprinting for identification of P.ternata.