Neurometabolic profiles of the substantia nigra and striatum of MPTP‐intoxicated common marmosets: An in vivo proton MRS study at 9.4 T |
| |
Authors: | Hwon Heo Jae‐Bum Ahn Hyeong Hun Lee Euna Kwon Jun‐Won Yun Hyeonjin Kim Byeong‐Cheol Kang |
| |
Affiliation: | 1. Department of Biomedical Sciences, Seoul National University, Seoul, South Korea;2. Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital, Seoul, South Korea;3. Graduate School of Translational Medicine, Seoul National University College of Medicine, Seoul, South Korea;4. Department of Radiology, Seoul National University Hospital, Seoul, South Korea;5. Institute of Radiation Medicine, Seoul National University Medical Research Center, Seoul, South Korea;6. Designed Animal and Transplantation Research Institute, Institute of GreenBio Science and Technology, Seoul National University, Pyeongchang, South Korea |
| |
Abstract: | Given the strong coupling between the substantia nigra (SN) and striatum (STR) in the early stage of Parkinson's disease (PD), yet only a few studies reported to date that have simultaneously investigated the neurochemistry of these two brain regions in vivo, we performed longitudinal metabolic profiling in the SN and STR of 1‐methyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐intoxicated common marmoset monkey models of PD (n = 10) by using proton MRS (1H–MRS) at 9.4 T. T2 relaxometry was also performed in the SN by using MRI. Data were classified into control, MPTP_2weeks, and MPTP_6‐10 weeks groups according to the treatment duration. In the SN, T2 of the MPTP_6‐10 weeks group was lower than that of the control group (44.33 ± 1.75 versus 47.21 ± 2.47 ms, p < 0.05). The N‐acetylaspartate to total creatine ratio (NAA/tCr) and γ‐aminobutyric acid to tCr ratio (GABA/tCr) of the MPTP_6‐10 weeks group were lower than those of the control group (0.41 ± 0.04 versus 0.54 ± 0.08 (p < 0.01) and 0.19 ± 0.03 versus 0.30 ± 0.09 (p < 0.05), respectively). The glutathione to tCr ratio (GSH/tCr) was correlated with T2 for the MPTP_6‐10 weeks group (r = 0.83, p = 0.04). In the STR, however, GABA/tCr of the MPTP_6‐10 weeks group was higher than that of the control group (0.25 ± 0.10 versus 0.16 ± 0.05, p < 0.05). These findings may be an in vivo depiction of the altered basal ganglion circuit in PD brain resulting from the degeneration of nigral dopaminergic neurons and disruption of nigrostriatal dopaminergic projections. Given the important role of non‐human primates in translational studies, our findings provide better understanding of the complicated evolution of PD. |
| |
Keywords: | 1‐methyl‐1 2 3 6‐tetrahydropyridine common marmoset MRS Parkinson's disease striatum substantia nigra |
|
|