Imaging porcine cardiac substrate selection modulations by glucose,insulin and potassium intervention: A hyperpolarized [1‐13C]pyruvate study |
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Authors: | Esben Søvsø Szocska Hansen Rasmus Stilling Tougaard Thomas Stokholm Nørlinger Emmeli Mikkelsen Per Mose Nielsen Lotte Bonde Bertelsen Hans Erik Bøtker Hans Stødkilde Jørgensen Christoffer Laustsen |
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Institution: | 1. MR Research Centre, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark;2. Danish Diabetes Academy, Odense, Denmark;3. Department of Cardiology, Aarhus University Hospital Skejby, Aarhus N, Denmark |
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Abstract: | Cardiac metabolism has received considerable attention in terms of both diagnostics and prognostics, as well as a novel target for treatment. As human trials involving hyperpolarized magnetic resonance in the heart are imminent, we sought to evaluate the general feasibility of detection of an imposed shift in metabolic substrate utilization during metabolic modulation with glucose–insulin–potassium (GIK) infusion, and thus the limitations associated with this strategy, in a large animal model resembling human physiology. Four 1‐13C]pyruvate injections did not alter the blood pressure or ejection fraction over 180 min. Hyperpolarized 1‐13C]pyruvate conversion showed a generally high reproducibility, with intraclass correlation coefficients between the baseline measurements at 0 and 30 min as follows: lactate to pyruvate, 0.85; alanine to pyruvate, 1.00; bicarbonate to pyruvate, 0.83. This study demonstrates that hyperpolarized 1‐13C]pyruvate imaging is a feasible technique for cardiac studies and shows a generally high reproducibility in fasted large animals. GIK infusion increases the metabolic conversion of pyruvate to its metabolic derivatives lactate, alanine and bicarbonate, but with increased variability. |
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Keywords: | cardiac GIK hyperpolarization MRI |
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