复方沙棘籽油栓对6种常见阴道乳杆菌增殖影响的体外研究

目的：研究复方沙棘籽油栓对6种常见阴道乳杆菌增殖的影响。方法：从中国健康妇女阴道分离乳杆菌进行纯化、鉴定后在体外培养，在培养基中加入不同浓度的复方沙棘籽油栓，观察该药对各菌种增殖的影响。结果：浓度≥405mg,／mL时，所有细菌无法生长；浓度在129mg／mL-405mg／mL时，对细菌产生抑制作用（P〈0．05）；当浓度在16．2mg／mL-64．8mg／mL时，对卷曲乳杆菌和加氏乳杆菌产生促进作用（P〈0．05），而对其它细菌无明显作用（P〉0．05）。结论：复方沙棘籽油栓对阴道乳杆菌各菌种的影响存在不同．且其影响与药物浓度有关。当药物浓度合适时．能对卷曲乳杆菌和加氏乳杆菌产生一定的促进作用。

Effect of Compound Hippophae Rhamnoides seed oil suppository on the proliferation of six species of vagi- nal Lactobacillus in vitro

Objectives： To study the effect of Compound Hippophae Rhamnoides seed oil suppository on proliferation of the six species of vaginal Lactobaeillus in vitro. Method： Vaginal Lactobacillus was isolated from va- ginal samples of healthy Chinese women, and was identified according to the 16s rRNA gene. Then the six identified species of vaginal lactobacillus were plated on the MRS culture medium including different concentration of Com- pound Hippophae Rhamnoides seed oil suppository, and anaerohieaUy cultured for a certain time. The diagrams of the colony were measured. Results： When the concentration of Compound Hippophae Rhamnoides seed oil supposi- tory was ≥405mg/mL, no Laetobacillus could grow. In the groups whose concentration of Compound Hippophae Rhamnoides seed oil suppository was between 129mg/mL and 405mg/mL, the diagrams of the colonies as to all the six species were shorter than those of the control group （ P 〈 0.05 ）. In the groups whose concentration of Compound Hippophae Rhamnoides seed oil suppository was between 16.2mg/mL and 64. 8mg/mL, the diagrams of the colonies of L. erispatus and L. gasseri were longer than those of the control group （ P 〈 0.05 ）, while the diagrams of the colonies of other species were similar to those of the control group （ P 〉 0. 05 ）. Condusion： The effect of Com- pound Hippophae Rhamnoides seed oil suppository on the proliferation of six species of vaginal laetobaeillus is relat- ed to the concentration, and it can facilitate the growth of L. erispatus and L. gassed to some extent when the con- centration is appropriate.