| GnRH及其受体在兔卵巢和子宫的分布 |
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| 引用本文： | 魏锁成,巩转娣,韦敏,谢坤. GnRH及其受体在兔卵巢和子宫的分布[J]. 免疫学杂志, 2010, 0(10) |
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| 作者姓名： | 魏锁成 巩转娣 韦敏 谢坤 |
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| 作者单位： | 西北民族大学生命科学与工程学院;西北民族大学医学院;兰州大学基础医学院; |
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| 基金项目： | 国家民委2007年度重点资助项目(MW2007-ZD-027) |
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| 摘 要： | 目的探讨GnRH与其受体(GnRHR)在卵巢和子宫组织中的分布,研究GnRH-A(激动剂Alarelin)主动免疫对GnRH和GnRHR分布的影响。方法 24只日本大耳白雌兔(Oryctolagus cuniculus)随机分为4组(n=6),实验组和对照组。其中实验1组(EG-1)、实验2组(EG-2)和实验3组(EG-3)分别在兔颈背侧皮下注射1.0m(l100μg/ml、100μg/ml和50μg/ml)GnRH-A抗原,EG-2和EG-3于20d以原剂量加强注射1次,102d无菌采集垂体、卵巢和子宫。用SP法染色,图像分析技术进行定位与分析。结果卵巢和子宫均有GnRH和GnRHR阳性细胞分布,主要见于卵母细胞、卵泡细胞、子宫内膜上皮细胞和腺上皮细胞;GnRH-A的剂量不同,GnRH和GnRH阳性细胞的染色强度也不同,即GnRHR和GnRH的表达量不同。GnRH-A能增加GnRH和GnRH的分布与表达,EG-2的作用更为明显。结论卵巢和子宫中均有GnRH和GnRHR细胞分布,GnRH-A免疫能增强GnRH和GnRHR的表达。
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| 关 键 词： | 促性腺激素释放激素 受体 免疫组织化学 |
Distribution of GnRH and GnRHR in ovary and uterus of female rabbits actively immunized with GnRH-A |
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| WEI Suocheng,GONG Zhu,i,WEI Min,XIE Kun Life Science , Engineering College,Northwest University for Nationalities,Lanzhou ,China. Distribution of GnRH and GnRHR in ovary and uterus of female rabbits actively immunized with GnRH-A[J]. Immunological Journal, 2010, 0(10) |
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| Authors: | WEI Suocheng GONG Zhu i WEI Min XIE Kun Life Science Engineering College Northwest University for Nationalities Lanzhou China |
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| Affiliation: | WEI Suocheng,GONG Zhuandi,WEI Min,XIE Kun Life Science and Engineering College,Northwest University for Nationalities,Lanzhou 730030,China |
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| Abstract: | The objectives of the present study is to study the distribution and location of gonadotropin-releasing hormone(GnRH) and its receptor(GnRHR) in ovaries and uterus of female rabbits,and to explore the effects of GnRH analogue(GnRH-A) on GnRH and GnRHR distribution.Twenty four female rabbits(Oryctolagus cuniculus) were randomly divided into EG-1,EG-2,EG-3 and control groups(each of 6 rabbits).Rabbits in EG-1,EG-2 and EG-3 groups were subcutaneously injected with 1.0 ml Alarelin antigen at concentration of 10... |
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| Keywords: | Gonadotropin-releasing hormone Receptor Immunohistochemistry |
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