| 细粒棘球绦虫重组Bb-Eg95-EgA31融合蛋白诱导小鼠免疫应答 |
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| 引用本文： | 周必英,陈雅棠,李文桂,杨梅. 细粒棘球绦虫重组Bb-Eg95-EgA31融合蛋白诱导小鼠免疫应答[J]. 免疫学杂志, 2010, 0(3) |
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| 作者姓名： | 周必英 陈雅棠 李文桂 杨梅 |
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| 作者单位： | 重庆医科大学附属第一医院传染病寄生虫病研究所;遵义医学院寄生虫学教研室; |
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| 基金项目： | 国家自然科学基金(30801052,30671835,30500423和30200239) |
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| 摘 要： | 目的研究细粒棘球绦虫(Eg)重组双歧杆菌(Bb)-Eg95-EgA31融合蛋白免疫小鼠后诱导的免疫应答。方法 56只SPF级雌性Balb/c小鼠随机均分7组,分别为重组Bb-Eg95-EgA31皮下注射组(A组)、肌肉注射组(B组)、鼻腔内接种组(C组)、口服灌胃组(D组)、空载体对照组(E组)、Bb对照组(F组)和Bb培养用液体培养基(MRS)对照组(G组)。免疫后8周各组鼠用50个Eg原头节攻击,攻击25周后,处死小鼠,分离细粒棘球蚴包囊并称重,计算囊重抑制率;ELISA检测血清IgG及其亚类和IgE和脾细胞上清液IL-12、IFN-γ、TNF-α和IL-10水平;MTT法测定脾细胞增殖反应;流式细胞仪检测脾CD4+和CD8+T细胞百分比和脾细胞凋亡发生率。结果重组Bb-Eg95-EgA31免疫组小鼠的囊重抑制率分别为45.33%、41.33%、70.67%和62.67%;血清IgG、IgG2a、IgG2b和IgG1水平升高,IgG3和IgE降低;脾IFN-γ、IL-12和TNF-α水平升高,IL-10水平降低;脾T淋巴细胞明显增殖;脾CD4+和CD8+T细胞显著增加;脾细胞凋亡发生率显著降低。结论细粒棘...
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| 关 键 词： | 细粒棘球绦虫 重组Bb-Eg95-EgA31 融合蛋白 免疫应答 |
Im mune response in mice induced by recombinant Bb-Eg95-EgA31 fusion protein of Echinococcus granulosus |
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| ZHOU Biying,CHEN Yatang,LI Wengui,YANG Mei Institute of Infectious , Parasitic Diseases,First Affiliated Hospital,Chongqing Medical University,Chongqing ,China. Im mune response in mice induced by recombinant Bb-Eg95-EgA31 fusion protein of Echinococcus granulosus[J]. Immunological Journal, 2010, 0(3) |
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| Authors: | ZHOU Biying CHEN Yatang LI Wengui YANG Mei Institute of Infectious Parasitic Diseases First Affiliated Hospital Chongqing Medical University Chongqing China |
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| Affiliation: | ZHOU Biying,CHEN Yatang,LI Wengui,YANG Mei Institute of Infectious and Parasitic Diseases,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China |
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| Abstract: | With the aim of setting a foundation for preparing vaccine against echinococcosis granulosus,we investigated the immune response in mice induced by recombinant Bifidobacteria bifidum(Bb)-Eg95-EgA31 fusion protein of Echinococcus granulosus(Eg).In this study,56 SPF Balb/c mice were randomly divided into 7 groups:recombinant Bb-Eg95-EgA31 subcutaneous injection group(group A),intramuscular injection group(group B),intranasal immunization group(group C),oral administration group(group D),blank vector control g... |
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| Keywords: | Echinococcus granulosus Recombinant Bb-Eg95-EgA31 Fusion protein Immune response |
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