细胞内Ca2+变化在砷剂诱导胰腺癌细胞株凋亡中的作用

目的：探讨细胞内游离Ca^2 在As2O3诱导的胰腺癌细胞株凋亡过程中的作用及Fas,Fas配体（FasL)的变化和意义。方法：2μmol/L的As2O3与胰腺癌细胞株SW－1990共同孵育后，H－E染色、光镜观察，抽提细胞DNA进行琼脂糖凝胶电泳，上流式细胞仪检测细胞凋亡特征； 用Fura-2荧光负荷技术测细胞内游离Ca^2 浓度（[Ca^2 ]i)，流式细胞仪检测Fas,FasL的表达情况。结果：2μmol/LAsO3作用于胰腺癌细胞48h后，胰腺癌细胞出现典型的凋亡特性改变，且凋亡过程中[Ca^2 ]i明显升高，Fas,FasL表达呈先升后降,结论：As2O3在诱导胰腺细胞凋亡过程中，肿瘤细胞凋亡与[Ca^2 ]i升高和Fas、FasL表达有关。

Effect of intracellular-free calcium changes on the process of pancreatic cancer cell line apoptosis induced by As2O3

Objective: To study the effect of intracellular-free calcium and the expression of Fas and Fas L on the process of pancreatic carcinoma cell apoptosis. Methods: Apoptosis induced by 2 μmol/L arsenic trioxide in pancreatic cancer cell lines SW-1990 was investigated.Concentration of intracellular-free calcium （［Ca2+］i） was determined by Fura-2a fluorescein load technique. Fas and FasL were determined by flow cytometry. Results: Pancreatic cancer cells treated with 2 μmol/L arsenic trioxide presented apoptotic features: intact cell membrane, chromatin condensation, nucleic fragmentation and apoptotic body formation; agarose electrophoresis showed marked ladder; flow cytometery analysis showed a sub-G1 cell peak. In the process of pancreatic carcinoma cell apoptosis Fas and FasL and the ［Ca2+］i were significantly higher than that in the control. Conclusion: The pancreatic cancer cell apoptosis induced by arsenic trioxide is related to Fas and FasL expression by the cancer cells and the ［Ca2+］i increase in the cancer cells.