肝细粒棘球蚴外囊壁钙化相关受体

目的 比较肝细粒棘球蚴病患者钙化外囊壁及非钙化外囊壁上的钙化相关受体BMPRⅡ(骨形态发生蛋白Ⅱ型受体)、IGF1R(胰岛素样生长因子1受体)和ERα(雌激素受体α)的表达差异。方法 钙化外囊壁和非钙化外囊壁茜素红染色,Envision免疫组化法和qRT-PCR分别检测同一细粒棘球蚴病患者钙化外囊壁及非钙化外囊壁上钙化相关受体BMPRⅡ、IGF1R和ERα的表达水平和钙化相关受体的mRNA表达量。结果 与细粒棘球蚴非钙化外囊壁相比较,同一患者钙化外囊壁茜素红染色钙化显著,且差异有统计学意义(χ²=20.369,P<0.01);钙化外囊壁相关受体的表达明显增加,且差异有统计学意义(P<0.05),mRNA表达量明显增高且差异具有统计学意义(P<0.05)。结论 肝细粒棘球蚴病患者钙化外囊壁钙化相关受体表达量较高,钙化相关因子通过与受体BMPRⅡ、IGF1R和ERα等结合,引起细粒棘球蚴外囊壁钙化,外囊壁的钙化可以有效地抑制细粒棘球蚴的生长,在细粒棘球蚴病患者临床治疗过程中发挥着重要作用。

Calcium related receptors of the pericyst of hepatic cystic echinococcosis

To compare the differential expression of BMPRⅡIGF1R and ERα between calcified hydatid pericyst wall and non-calcified hydatid pericyst wall, we detected the expression of BMPRⅡIGF1R and ERα in calcified pericyst wall and non-calcified pericyst wall of hepatic cystic echinococcosis respectively with Alizarin Red stainingenvision immuno-histochemical method and qRT-PCR. In comparison with non-calcified hydatid pericyst wall, the red calcified nodes of Alizarin Red staining on the calcified hydatid pericyst wall were obviously increased, and differences were statistically significant (χ2=20.369, P<0.01). The expression levels of BMPRⅡIGF1R and ERα were much higher in calcified hydatid pericyst wall than those in non-calcified hydatid pericyst wall with statistical significance (P<0.05). Compared with control groups, the mRNA relative expression levels of BMPRⅡIGF1R and ERα were statistically higher in calcified pericyst wall group (P<0.05). The expression levels of these calcium related receptor were higher in calcified hydatid pericyst wall than those in non-calcified hydatid pericyst wall. Cytokines binding with these calcium related receptors may contribute to the response; calcified hydatid pericyst wall may inhibit Echinococcus granulosus and plays an important role on treatment of hepatic cystic echinococcosis.