尼美舒利对肝癌SMMC-7721细胞增殖、凋亡的干预作用

目的 :研究尼美舒利对肝癌SMMC 772 1细胞增殖、凋亡的干预作用。方法 :研究对象为SMMC 772 1肝癌细胞系 ,细胞增殖采用MTT比色法检测 ,细胞凋亡采用电镜观察、流式细胞仪和TUNEL法检测。结果 :尼美舒利显著抑制体外培养的SMMC 772 1细胞增殖 ,随着药物剂量的增加和时间的延长 ,SMMC 772 1的生长、增殖明显抑制 ,呈剂量和时间依赖性。流式细胞术、TUNEL法检测示 ,使用不同浓度尼美舒利作用于SMMC 772 1细胞 72h后 ,可诱导SMMC 772 1细胞凋亡 ,随着药物浓度增加 ,凋亡率和凋亡指数均显著增加 ,差异有显著性。结论 :选择性COX 2抑制剂尼美舒利可以显著抑制SMMC 772 1细胞增殖 ,且呈明显的时间、剂量依赖关系 ,同时诱导SMMC 772 1细胞凋亡 ,且具有剂量依赖性

The Effect of Nimesulide on Proliferation and Apoptosis of SMMC-7721 Cells

Objective: To investigate the effect of Nimesulide on proliferation and apoptosis of SMMC 7721 cells. Methods: The study was carried out on the culture of hepatic carcinoma cell line SMMC 7721 cells. Various concentrations of Nimesulide were added and incubated. Cell proliferation was detected with MTT colorimetric assay. Cell apoptosis was detected by electron microscopy, flow cytometry and TUNEL.Results: Nimesulide could significantly inhibit SMMC 7721 cells proliferation does dependently and time dependently compared with the control group. After Nimesulide incubation for 72 h,the apoptosis rate and apoptosis index of SMMC 7721 cells were higher than those in control group (P<0.05). Conclusion: The selective COX 2 inhibitor, Nimesulide could inhibit proliferation of SMMC 7721 cells and increase apoptosis rate and apoptosis index of SMMC 7721 cells.