| 固相萃取反相高效液相色谱法测定环孢素A滴眼剂中环孢素A的含量 |
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| 引用本文： | 赵虹,荣墨克,郎志刚,薄立华,冀志敏.固相萃取反相高效液相色谱法测定环孢素A滴眼剂中环孢素A的含量[J].吉林医学,2003,24(5):436-437. |
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| 作者姓名： | 赵虹 荣墨克 郎志刚 薄立华 冀志敏 |
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| 作者单位： | [1]吉林大学中日联谊医院，吉林长春130031 [2]吴太药业集团，吉林长春130021 |
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| 摘 要： | 目的:应用固相萃取改进环孢素A滴眼剂中环孢素A含量的测定方法。方法:环孢素A滴眼剂中的环孢素A经固相萃取,以反相高效液相色谱法测定。色谱柱Waters Sep—Pak C18萃取小柱,Nova—Pak C18分析柱;流动相乙腈:甲醇:水(265:22:120);流速0.5ml/min;检测波长206nm。结果:该法回收率为98.6％,相对标准差(RSD)为2.4％,线性范围为0.5-40μg/ml,r=0.9993。结论:该法快速、敏感、高效,可用于环孢素A滴眼剂中环孢素A含量的测定。
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| 关 键 词： | 固相萃取 反相高效液相色谱法 环孢素A 滴眼剂 |
| 文章编号： | 1004-0412(2003)04-0436-02 |
| 修稿时间： | 2003年4月29日 |
Determination of CsA in eye drops by RP-HPLC coupled to solid-phase extraction |
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| ZHAO Hong,RONG Mo-ke,LANG Zhi-gang,BO Li-hua,JI Zhi-min.Determination of CsA in eye drops by RP-HPLC coupled to solid-phase extraction[J].Jilin Medical Journal,2003,24(5):436-437. |
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| Authors: | ZHAO Hong RONG Mo-ke LANG Zhi-gang BO Li-hua JI Zhi-min |
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| Abstract: | Objective To improve an analytical method of CsA in eye drops by solid-phase extraction. Method The CsA in eye drops was processed by Waters Sep-Pak solid-phase extractor, and the column was Waters Nova-Pak C18. The mobile phase was acetonitrile:methanol:water (265:22:120),flow rate was 0.5ml/min, the detective wavelength was 206nm. Results The average recovery rate was 98.6%, the relative standard deviation was 2.4%, the linearity range was 0.5-40ug/ml, the coefficient of correlation was 0.9993. Conclusions The method is rapid,sensitive and efficient for determination of CsA in eye drops. |
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| Keywords: | Solid-phase extraction RP-HPLC CsA Eye drops |
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