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| 负性协同刺激分子B7-H4在小鼠树突状细胞上的表达及其生物学意义 | |
| 引用本文： | 瞿秋霞,陈成,蒋玉平,陈曦,沈宇,朱一蓓,张学光.负性协同刺激分子B7-H4在小鼠树突状细胞上的表达及其生物学意义[J].中华微生物学和免疫学杂志,2009,29(10). |
| 作者姓名： | 瞿秋霞 陈成 蒋玉平 陈曦 沈宇 朱一蓓 张学光 |
| 作者单位： | 1. 苏州大学附属第一医院临床免疫学实验室,215006 2. 苏州大学医学生物技术研究所,215007 3. 215006,苏州大学附属第一医院临床免疫学实验室;215007,苏州大学医学生物技术研究所 |
| 基金项目： | 国家重点基础研究发展规划(973计划)资助项目，国家自然科学基金资助项目，苏州大学附属第一医院科研项目 |
| 摘 要： | 目的 探讨B7-H4分子在小鼠树突状细胞分化发育过程中的表达及其在T细胞活化中的作用.方法 采用GM-CSF和IL-4联合方案体外诱导小鼠髓系树突状细胞(DCs);采用流式细胞术检测未成熟DCs、成熟DCs以及IL-10诱导的DCs表面B7-H4分子的表达;采用3H-TdR掺入试验和抗B7-H4单抗(McAb)阻断试验分析DCs表达的B7-H4分子对T淋巴细胞的共刺激效应.结果 经GM-CSF和IL-4联合方案体外诱导的未成熟DCs较高水平表达B7-H4,在IL-10作用下B7-H4表达进一步上调,经TNF-α刺激成熟后,B7-H4的表达显著下调,不同功能状态下DCs表面B7-H4分子均可抑制T细胞的增殖,但以未成熟DCs、IL-10诱导的DCs表面B7-H4分子的抑制作用更为显著.结论 不同功能状态下的DCs均有B7-H4分子的表达,处于抑制状态下的DCs通过高表达B7-H4介导免疫不应答效应. |
| 关 键 词： | 树突状细胞 T淋巴细胞 |
Expressions of B7-H4 in mouse myeloid dendritic cells and its role in activation of T lymphocytes |
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| Abstract: | Objective To study the role of B7-H4 on the surface of murine DCs in mediating T cell activation.Methods Mouse myetoid dendritic cells(DCs)were generated from bone marrow in vitro using GM-CSF and IL-4,and further stimulation with TNF-α or IL-10 for 48 h,respectively.Expressions of B7-H4 on DCs were analyzed by FCM.3H-thymidine incorporation test was used to detect the T cell proliferation stimulated by DCs with or without blocking B7-H4 by McAb.respectively.Results 5-6 d DCs in our system can be regarded as imDCs.B7-H4 was expressed moderately on imDCs,and could be markedly upregulated by IL-10,and be markedly down-regulated by TNF-α.3H-thymidine incorporation test showed that blockade of B7-H4 on imDCs and IL-10-treated DCs resulted in enhancement of T cell proliferation signiticantly.When T cells encountered with mDCs.B7-H4 blockade could not markedly enhance the T cells proliferation as seen in inhibitory DCs.Conclusion Expression of B7-H4 on inhibitory DCs might contribute to its immunoinhibitory effect. |
| Keywords: | B7-H4 |
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