A simple method for the preparation of liposomes for pharmaceutical applications: characterization of the liposomes. |
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Authors: | S Perrett M Golding W P Williams |
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Affiliation: | Biomolecular Sciences Division, King's College London, UK. |
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Abstract: | A new method for the preparation of liposomes is described that avoids the use of pharmaceutically unacceptable solvents and energy-expensive procedures such as sonication. The method is based on the initial formation of a proliposome mixture containing lipid, ethanol and water, which is converted to lipsomes by a simple dilution step. Measurements using 6-carboxyfluorescein as a marker indicate that water-soluble drugs can be trapped with extremely high efficiency (65-80% depending on lipid composition). The structural organization of the proliposome mixture and the final liposomes were characterized using electron microscopy and 31P-NMR. |
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