红景天苷对重症急性胰腺炎大鼠胰腺细胞自噬的影响

目的:研究红景天苷（salidroside，Sal）对重症急性胰腺炎大鼠胰腺细胞自噬的影响。方法:50只雄性Wistar大鼠随机（随机数字法）分为假手术组（Sham组）、重症急性胰腺炎组（severe acute pancreatitis, SAP组）、红景天苷15 mg/kg治疗组（Sal 15组）、红景天苷30 mg/kg治疗组（Sal 30组）和红景天苷60 mg/kg治疗组（Sal 60组）五组，每组各10只。Sham组采用胰管内逆行注射生理盐水，SAP组和Sal各剂量组均采用胰管内逆行注射5%牛胆酸钠建立模型。Sal各剂量组于造模后2 h分别腹腔注射上述不同浓度红景天苷溶液。酶联免疫吸附法（enzyme-linked immunosorbent assay, ELISA）检测血清淀粉酶(amylase, AMY)活性和血浆肿瘤坏死因子-α(tumour necrosis factor-α, TNF-α)水平，Western blot检测胰腺组织蛋白表达水平，光学显微镜观察胰腺组织病理变化。实验数据采用SPSS 24.0进行分析。组间比较采用单因素方差分析，以 P<0.05为差异有统计学意义。 结果:大鼠经造模后红景天苷干预，TNF-α（28.1±0.9）pg/mL、p65蛋白表达水平（0.52±0.01）和AMY活性（3 801±292） U/L在Sal 60组最低，组间差异有统计学意义（ P<0.05）；p-mTOR水平在Sal 60组最低，LC3水平在Sal 60组最高（ P<0.05）；且随着红景天苷剂量增加，胰腺组织病理损伤程度呈下降趋势。 结论:红景天苷对重症急性胰腺炎大鼠发挥保护作用，且以Sal 60组剂量作用最明显。

Effect of salidroside on autophagy of pancreatic cells in rats with severe acute pancreatitis

Objective:To study the effects of salidroside (Sal) on autophagy of pancreatic cells in rats with severe acute pancreatitis (SAP).Methods:Fifty healthy male Wistar rats were randomly (random number) divided into the sham group, SAP group, Sal 15 group, Sal 30 group and Sal 60 group, 10 rats in each group. The sham group was injected retrogradely normal saline into pancreatic duct, while the 5% sodium taurocholate was injected retrogradely into pancreatic duct to establish severe pancreatitis model in the SAP group and all the Sal groups. Salidroside at different doses were intraperitoneally injected in all the Sal groups at 2 h after surgery. The serum amylase (AMY) activity and plasma tumour necrosis factor-α (TNF-α) level were detected by enzyme-linked immunosorbent assay (ELISA), and Western blot was used to detect the expression of pancreatic tissue protein. The histopathological changes of pancreas were observed under the light microscope. The data were analyzed by SPSS 24.0, and One-way ANOVA was used for comparison between groups. A P value less than 0.05 was considered to be statistically significant. Results:After salidroside intervention, the levels of TNF-α (28.1±0.9) pg/mL, the relative expression of p65 (0.52±0.01), AMY activity (3 801±292) and relative expression of p-mTOR were the lowest in the Sal 60 group (all P<0.05); while the relative expression of LC3 was the highest in the Sal 60 group (all P <0.05). The degree of pathological damage of pancreatic tissues was decreased with the increase of salidroside dose. Conclusions:Salidroside at different doses have protective effects on rats with severe pancreatitis, which seems to be most obvious in the Sal 60 group.