Bcl-2、C-ras p21和PCNA在卵巢上皮性囊腺肿瘤的表达及意义

目的　探讨Bcl 2、C rasp2 1和增殖细胞核抗原 (PCNA)在卵巢上皮性肿瘤的表达及意义。方法　应用免疫组织化学ABC法对卵巢囊腺癌 6 0例、交界性囊腺瘤及良性囊腺瘤各 30例 ,进行Bcl 2、C rasp2 1和PCNA的检测。结果　Bcl 2、C rasp2 1在卵巢囊腺癌、交界性囊腺瘤及良性囊腺瘤的表达分别为 6 3 .3%和 81.5 %、37.0 %和 6 0 .6 %及 6 .6 %和 17.2 % ,三者之间差异具有显著性 (P <0 .0 1)。结论　检测C rasp2 1的表达率及PCNA的细胞增殖分数对判断卵巢囊腺瘤的良恶性及分级具有重要意义。C rasp2 1及PCNA表达强阳性者平均生存期较短 ,并与转移、扩散有关。Bcl - 2的表达与分化程度、预后有关 ,与转移、腹水形成无关

Expression of bcl-2 ,C-ras p21 and PCNA in ovarian epithelial cystadenoma

Objective To investigate expressions of concogene bcl-2,C-rasp21 and proliferating cell nuclear antigen (PCNA) in ovarian epithelial cystadenoma. Methods The expressions of bcl-2, C-rasp21 and PCNA were detected by ABC immunohistochemical method in 60 patients with cystadenocarcinoma,30 borderline cystadenoma and 30 benign cystadenoma. Results Positive expression of bcl-2, C-rasp21 in cystadenocarcinoma,borderline and benign cystadenoma were 63.3% and 81.5%, 37.0% and 60.6%, 6.6% and 17.2%, respectively. There were significant difference among three group. Conclusion The expression of C-rasp21 combined cellular proliferation of PCNA could help diagnose benign or malignant ovarian cystadenomas. Both strong positive expression of C-rasp21 and PCNA may mean shorten survival period, tumor metastasis and diffusion. The expression of bcl-2 was correlated with degree of differentiation and prognosis, but was not related to tumor metastasis and ascites.