西藏门巴族人群HLA-A、B和DRB1基因座多态性

目的 分析西藏门巴族HLA-A、B和DRB1 3个基因座的遗传特征，并研究其民族起源。方法应用序列特异性寡核苷酸探针反向斑点杂交技术对西藏自治区门巴族居住区47名门巴族无关个体HLA-A、B和DRB1基因座进行分型。对门巴族和我国其他11个群体(民族)的HLA-DRB1基因频率采用Neighbor-Joining(NJ)方法进行聚类分析。结果 在西藏门巴族人群中HLA-A基因座共检出23个等位基因，其中HLA-A*1101，A*2402，A*02011，A*0206基因最常见；HLA-B基因座共检出39个等位基因，其中HLA-B*3802，B*4001，B*4002，B*51011基因最常见；HLA-DRB1基因座共检出33个等位基因，其中HLA-DRB1*12021，DRB1*0403，DRB1*0701，DRB1*1201基因最常见，频率最高的等位基因分别是HLA-A*1101(0．2128)，HLA-B*3802(0．1064)和HLA-DRB1*12021(0．0851)。结论 西藏门巴族人群HLA基因座具有高度遗传多态性，聚类分析门巴族与西藏藏族遗传关系较近。

Determination of HLA-A, B, and DRB1 gene polymorphism in Monba ethnic group of Xizang Autonomous Region, China

Objective To investigate the HLA-A, B, DRB1 a llele polymorphism in Monba ethnic population of Xizang Autonomous Region. Methods HL A-A, B, DRB1, DNA types in 47 unrelated Monba ethnic healthy individuals from t h e Xizang Autonomous Region of China were analyzed by polymerase chain reaction- based reverse line-strip sequence specific oligonucleotide hybridization. Based on the HLA-DRB1 alleles frequencies of 11 other ethnic group s in China, a molecular phylogenetic tree was constructed by the Neighbor-Joini ng (NJ) method. Results Twenty-three alleles were detected for HLA-A, 39 for HLA-B, and 33 for HLA-DRB1. HLA-A*1101, A*2402, A*02011,A*02 06, HLA-B*3802,B*4001,B*4002, B*51011, HLA-DRB1*12021,DRB1*0403,DRB1*0701, DRB1*1201 were the most common alleles. The most frequent alleles were HLA-A*1 1 01(0.2128), HLA-B*3802(0.1064), and HLA-DRB1*12021(0.0851). Conclusion The HLA loci are highly polymorphic in Monba population of Xizang Auto nomous Region. Compared with other ethnic groups in China, the Monba ethnic grou p is close to Tibetan ethnic group.