胶原蛋白-几丁聚糖三维骨架构建组织工程血管的可行性

目的 研究胶原蛋白——几丁聚糖构建的三维支架在血管组织工程研究中的可行性。方法 以Ⅰ型胶原蛋白和几丁聚糖为生物材料，体外预构具有三维结构的支架，观察支架的空间立体结构；将培养的种子细胞（人平滑肌细胞和纤维细胞）种植于支架，观察材料与细胞的生物相容性和抗收缩性，并测定材料中DNA含量和胶原蛋白含量。结果 应用相分离技术制备的支架，具有均匀的三维结构和丰富的交通孔，种植种子细胞后可以形成良好的细胞黏附和生长，且选用的支架材料可以抵抗细胞收缩引起的材料变形；培养2、4、6周的材料中，DNA含量和胶原蛋白含量随培养时间延长而明显增加。结论构建的胶原蛋白——几丁聚糖三维支架具有结构均匀、细胞生物相容性好的特点，并具有一定的物理强度，可以应用于血管组织工程研究。

Feasible Application of Collagen-Chitosan 3-D Scaffolds in Tissue Engineering Blood Vessel Research

Objective To study feasible application of collagen-chitosan 3-dimensional(3-D) scaffolds to tissue enginee-ring blood vessel research. Methods Collagen-chitosan was used as the biomaterial to construct 3-D scaffolds, human smooth muscle cells (SMCs), and fibroblasts were proliferated as seed cells in vitro and seeded on the scaffolds to determine these biocompatibility and anti-contractibility. The 3-D structure was observed, and DNA and collagen contents in cultured scaffolds were measured. Results The scaffolds fabricated by phase separation technique had relatively uniform construction, with plenty of traffic pores, and could facilitate seed cell adherence and growth, more ever they also could hold the 3-D form to stand against cells contraction. The contents of DNA and collagen in the scaffolds cultured for 2, 4, 6 weeks were increased significantly in a time-dependent manner.Conclusion The scaffolds made from collagen-chitosan, with 3-D structure, cell biocompatibility and good mechanical strength, can be used in tissue engineering blood vessel research.