Improved assay for monocyte chemotaxis using frozen stored responder cells |
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Authors: | David A. Dean Douglas M. Strong |
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Affiliation: | Department of Clinical and Experimental Immunology, Naval Medical Research Institute, Bethesda, Maryland 20014, U.S.A. |
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Abstract: | Frozen stored human mononuclear cells were compared with non-frozen cells as responder cells in the monocyte chemotaxis assay. Frozen stored cells were found to exhibit lower background migration, resulting in greater experimental-to-control ratios. The use of frozen cells also greatly improved the speed, standardization of conditions and day-to-day reproducibility of the assay procedure. Frozen cells were at least as sensitive as fresh cells for detecting low chemotactic factor concentrations and were superior for detecting differences in concentration. |
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