|
|||||
|
|
Serological precipitation method for studying biosynthesis and secretion of immunoglobulins by human peripheral blood lymphocytes |
|
| Authors: | Yong Sung Choi |
| Affiliation: | Memorial Sloan-Kettering Cancer Center, Walker Laboratory, Rye, NY 10580, U.S.A. |
| Abstract: | A reproducible, serological method to quantify radiolabeled immunoglobulin (Ig) synthesized and secreted by human peripheral blood lymphocytes is described. In contrast to indirect precipitation (rabbit antihuman Ig and goat antirabbit Ig), the direct coprecipitation method using human IgG and rabbit antihuman IgG F(ab)2 showed much smaller, non-specifically precipitable radioactivity (i.e., 2～14%) without affecting Ig radioactivity. |
| Keywords: | PBL peripheral blood lymphocytes FCS fetal calf serum NP-40 Nonidet P-40 TCA trichloroacetic acid PBS phosphate buffered saline KLH keyhole limpet hemocyanin SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
| 本文献已被 ScienceDirect 等数据库收录！ | |