弓形虫慢性感染大鼠脑组织中Toll样受体4基因表达及其对脑损害影响

目的 目的 探讨Toll 样受体4 （TLR4） 在弓形虫慢性感染大鼠脑组织中的表达, 及其对脑部损害的作用。 方法 方法 将10 只SD雄性大鼠随机分为对照组和模型组, 每组5只。感染组每鼠腹腔感染1×107 /ml弓形虫速殖子2 ml, 对照组每鼠腹腔注射灭菌生理盐水2 ml。弓形虫感染后10周, 用ELISA法检测大鼠血清炎症因子IL?1β和IL?4水平; 采用逆转录多聚酶链反应 （RT?PCR） 法检测大鼠脑组织TLR4 mRNA的表达。 结果 结果 与正常对照组相比, 弓形虫慢性感染组大鼠脑TLR4 mRNA 表达显著升高 （P＜0.05）, 血清IL?1β含量显著上升 （P＜0.05）, IL?4含量也有所升高, 但差异无统计学意义 （P＞0.05）。 结 结论 论 TLR4在弓形虫慢性感染大鼠的脑部损害中具有一定作用。

Expression of Toll-like receptor 4 in brain tissue of chronic Toxoplasma gondii infection rats and its effect on brain injury

Objective To explore the expression of Toll-like receptor 4 (TLR4) in brain tissue of chronic Toxoplasma gondii infection rats and its effect on brain injury. Methods Ten male SD rats were randomly divided into 2 groups, namely control and infection groups. Each rat in the infection group was intraperitoneal injected with Toxoplasma gondii tachyzoites 107/ml × 2 ml, and that in the control group was injected with 2 ml sterile normal sodium. After 10 weeks, the expression of TLR4 mRNA in the brain tissue was determined by RT-PCR, and the levels of IL-1β and IL-4 in peripheral blood sera were detected by ELISA. Results Compared with the control group, the expression of TLR4 gene and the peripheral blood serum level of IL-1β of rats in the Toxoplasma gondii infection group were both significantly increased, with all P values were less than 0.05, and the level of IL-4 was also increased, but the difference had no statistically significance (P>0.05). Conclusion TLR4 might be involved in inflammatory reactions of brain injury for chronic Toxoplasma gondii infection rats.