HPLC法测定十滴水中五种活性成分的含量

目的：建立HPLC法同时测定十滴水中的芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法。方法：色谱柱为ShimadzuVP-ODS柱（4.6mm×250mm,5μm）,流动相为乙腈-0.1%磷酸梯度洗脱,流速为1.0ml/min,检测波长为254nm,柱温30℃。结果：5种成分分别在0.042～0.840μg（r=0.9996）、0.168～3.352μg（r=0.9998）、0.084～1.680μg（r=0.9997）、0.093～1.852μg（r=0.9999）和0.174～3.490μg（r=0.9994）内呈良好线性关系,平均回收率分别为99.60%（RSD=0.20%）、99.56%（RSD=0.36%）、98.95%（RSD=0.55%）、98.76%（RSD=0.97%）和98.79%（RSD=1.08%）。结论：本法简便、快速、准确,可用于十滴水的质量控制。

Determination of five effective constituents in Shidishui by HPLC

Objective： To establish a method for the simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Shidishui by HPLC. Methods： The sample was separated on a Shimadzu VP-ODS （4.6 mm×250 mm, 5.0 μm） with acetonitrile-0.1% phosphoric acid in gradient elution. The flow rate was 1.0 ml/min, the detection wavelength was 254 nm and the column temperature was 30℃. Results： Aloe-emodin, rhein, emodin, chrysophanol and physcion had good linear relationship in the range of 0.042-0.840 μg （r=0.999 6）, 0.168-3.352 μg （r=0.999 8）, 0.0841.680 μg （r=0.999 7）, 0.093-1.852 μg （r=0.999 9） and 0.174-3.490 μg （r=0.999 4）. Their average recoveries were 99.60% （RSD=0.20%）, 99.56% （RSD=0.36%）, 98.95% （RSD=0.55%）, 98.76% （RSD=0.95%）, and 98.79% （RSD=1.08%）, respectively. Conclusion： The method is rapid, simple, accurate, and applicable for the quality control of Shidishui.