| 抗人甲状腺球蛋白单克隆抗体的研制和初步应用 |
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| 引用本文： | 沈茜,李友发,徐登仁,曹文飞,李吉娜. 抗人甲状腺球蛋白单克隆抗体的研制和初步应用[J]. 现代免疫学, 1985, 0(5) |
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| 作者姓名： | 沈茜 李友发 徐登仁 曹文飞 李吉娜 |
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| 作者单位： | 第二军医大学附属长海医院实验科，第二军医大学附属长海医院实验科，第二军医大学附属长海医院实验科，第二军医大学附属长海医院实验科，第二军医大学附属长海医院实验科 进修生 |
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| 摘 要： | 本文采用体细胞杂交瘤技术和酶联免疫吸附试验筛选技术,获得了6株持续分泌抗人甲状腺球蛋白 McAb 的杂交瘤细胞系。这6种 McAb 对人 T_3/T_4、及家兔、小牛、大鼠的甲状腺球蛋白均无交叉反应,但其中4种 McAb 对狗甲状腺球蛋白有不同程度的交叉反应,包括2种 McAb对人甲状腺微粒体也有交叉反应,余2种 McAb 均无交叉反应。将所得杂交瘤细胞接种 BALB/c鼠腹腔,获得高效价的腹水 McAb。本文还以这些 McAb 用免疫组化法,研究和检测病理甲状腺组织中甲状腺球蛋白的可能性进行了初步探索,并讨论了其临床意义。
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PRODUCTION AND PRIMARY APPLICATION OF MONOCLONAL ANTIBODIES TO HUMAN THYROGLOBULIN |
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| Abstract: | The production of six hybridoma cell lines secreted monoclonal antibodies(McAb)to human thyroglobulin(TG)by hybridoma technique and enzyme-linked immunosorbent assay was reported.The specificity of these McAbs was demonstrated by the absence of cross-reaction by human T_3,T_4,and TG of rabbit,bovine,rat.The four McAbs were found cross-reaction with dog's TG and others did not.Six hybridoma cell lines could produce ascitic fluids with high titer McAb after being inoculated into BALB/C mice I.P. Histochemical staining was used on normal and neoplastic thyroid-gland tissues with these McAbs,and the clinical significance of this method was briefly discussed. |
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