HPLC法同时测定山楂异槲皮苷和金丝桃苷含量

目的采用HPLC法测定市售山楂中异槲皮苷及金丝桃苷的含量以建立质量控制方法。方法采用Ultimate@XB-C18色谱柱,采用梯度洗脱条件,检测波长:360 nm,流速:1 mL/min,柱温:30℃,进样量:10μL,对山楂中异槲皮苷、金丝桃苷进行检测,并进行系统适用性考察。结果经方法学验证,线性范围分别为0.60~38.52μg/m L、0.60~38.56μg/m L,相关系数均高于0.999;精密度、重复性的RSD均小于2%;平均回收率101.88%~102.44%,RSD小于2.5%。结论本法快速准确,可用于市售山楂异槲皮苷和金丝桃苷的含量测定,为其质量控制提供试验依据。

Simultaneous Determination of Isoquercetin and Hyperoside in Hawthorn by HPLC

Objective In order to establish the quality control method, isoquercitrin and hyperoside content of commercially available hawthorn were determined by HPLC. Methods When the Ultimate@XB-C18 column with different mobile phase elution conditions, detection wavelength: 360 nm, flow rate: 1 mL/min, column temperature:30 ℃, sample volume: 10 μL, the isoquercitrin and hyperoside of commercially available fresh hawthorn and the crude slices were detected, and the system suitability was studied. Results Then a study was given to verify the established method, the results showed that the linear range of isoquercitrin and hyperoside were 0.60-38.52 μg/mL and 0.60-38.56 μg/m L;the correlation coefficients were higher than 0.999;and the precision and repeatability of RSD were smaller than 2%, the recovery rate was 101.88%-102.44%, RSD less than 2.5%. Conclusion The method was rapid, simple, accurate, reliable and could be used to detect the isoquercitrin and hyperoside of hawthorn.