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| CD4+CD25+调节性T细胞对人外周血内皮祖细胞增殖、迁移、黏附的影响 | |
| 引用本文： | 谢培益,苏又苏,汤海燕,方红成,何少林,李大主. CD4+CD25+调节性T细胞对人外周血内皮祖细胞增殖、迁移、黏附的影响[J]. 临床心血管病杂志, 2011, 27(1) |
| 作者姓名： | 谢培益 苏又苏 汤海燕 方红成 何少林 李大主 |
| 作者单位： | 1. 广东医学院附属深圳南山人民医院心内科,广东深圳,518052 2. 深圳市职业病防治院检验科 3. 华中科技大学同济医学院协和医院心内科 |
| 摘 要： | 目的:研究CD4+CD25+调节性T细胞对体外培养的人外周血内皮祖细胞(EPCs)增殖、迁移、黏附的影响。方法:密度梯度离心法分离培养人外周血单个核细胞,经FITC-UEA-I和Dil-acLDL双染色鉴定为正在分化的EPCs。进一步采用流式细胞仪检测其表面标志CD34、CD133。磁性细胞分离器(MACS)分离CD4+CD25+调节性T细胞及CD4+CD25-T细胞。将EPCs分别与CD4+CD25+调节性T细胞或CD4+CD25-T细胞共培养36h。采用MTT比色法、transwell小室、细胞计数法观察EPCs增殖、迁移、黏附能力。结果:与对照组和CD4+CD25-T细胞相比,与CD4+CD25+调节性T细胞共培养EPCs增殖、迁移、黏附能力显著增强,且呈浓度依赖性增强。结论:CD4+CD25+调节性T细胞可显著促进EPCs增殖、迁移、黏附能力,此作用可能是其抗AS作用机制之一。 |
| 关 键 词： | 动脉粥样硬化 调节性T细胞 内皮祖细胞 增殖 迁移 黏附 |
The effect of CD4+CD25+ regulatory T cells on the proliferation,migration and adhesion of endothelial progenitor cells |
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| XIE Peiyi,SU Yousu,TANG Haiyan,FANG Hongcheng,HE Shaolin,LI Dazhu. The effect of CD4+CD25+ regulatory T cells on the proliferation,migration and adhesion of endothelial progenitor cells[J]. Journal of Clinical Cardiology, 2011, 27(1) | |
| Authors: | XIE Peiyi SU Yousu TANG Haiyan FANG Hongcheng HE Shaolin LI Dazhu |
| Affiliation: | XIE Peiyi1 SU Yousu1 TANG Haiyan2 FANG Hongcheng1 HE Shaolin3 LI Dazhu3(1Department of Cardiology,The NanShan People's Hospital Affiliated to Guangdong Medical College,Shenzhen,518052,China,2Clinical Labolatory,shenZhen Hospital for the Prevention and Treatment of Occupational Diseases,3Institute of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology) |
| Abstract: | Objective:To investigat the effect of CD4+CD25+ regulatory T cells(Tregs) on the proliferation,migration and adhesion of endothelial progenitor cells(EPCs) from human circulating blood in vitro.Method:Tregs were isolated from lymphocyte suspensions by magnetic cell sorting column and analyzed by flow cytometry.Human blood mononuclear cells were isolated with Ficoll by density gradient centrifugation.EGM-2MV culture fluid was added,and then cells were plated on dishes coated with human fibronectin.After 7 da... |
| Keywords: | atherosclerosis CD4 CD25 Tregs endothelial progenitor cells proliferation migration adhesion |
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