| 紫杉醇对卵巢癌细胞基质金属蛋白酶-2、9基因表达的影响 |
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| 引用本文： | 张爱武,张苏江,姜藻. 紫杉醇对卵巢癌细胞基质金属蛋白酶-2、9基因表达的影响[J]. 现代妇产科进展, 2002, 11(5): 331-333 |
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| 作者姓名： | 张爱武 张苏江 姜藻 |
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| 作者单位： | 1. 南京医科大学附属南京第一医院妇产科,南京,210006
2. 东南大学附属中大医院肿瘤科 |
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| 摘 要： | 目的:研究紫杉醇对HO8910人卵巢癌细胞的体外杀伤作用;紫杉醇对MMP-2、MMP-9 mRNA表达的影响。方法:采用MTT法检测紫杉醇对HO8910细胞的抑制率;采用半定量RT-PCR法检测紫杉醇作用于HO8910细胞时MMP-2、MMP-9基因mRNA水平的变化。结果:紫杉醇在达10-7mol/L以上浓度时对HO8910细胞抑制率>30％(P<0.01);10-8mol/L以上浓度时紫杉醇可下调MMP-2、MMP-9 mRNA的表达,其作用呈剂量依赖性。结论:在10-7mol/L以上浓度,HO8910细胞对紫杉醇具有药物敏感性;紫杉醇可能通过抑制MMP-2、MMP-9 mRNA的表达,抑制肿瘤的侵袭及转移。
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| 关 键 词： | 金属蛋白酶 紫杉醇 卵巢肿瘤: 细胞系,HO8910 基因表达 |
| 文章编号： | 1004-7379(2002)05-0331-03 |
| 修稿时间： | 2002-04-20 |
The influence of paclitaxel on matrix metalloproteinase-2,9 expression in ovarian carcinoma cells |
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| Zhang Aiwu,Zhang Sujiang,Jiang Zao.. The influence of paclitaxel on matrix metalloproteinase-2,9 expression in ovarian carcinoma cells[J]. Current Advances In Obstetrics and Gynecology, 2002, 11(5): 331-333 |
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| Authors: | Zhang Aiwu Zhang Sujiang Jiang Zao. |
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| Affiliation: | Zhang Aiwu,Zhang Sujiang,Jiang Zao. 1. Department of Obstetrics and Gynecology,TheAffiliated Nanjing First Hospital of Nanjing Medical University,Nanjing 210000, 2. Department of Phymatology,Zhongda Hospital of Dongnan University |
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| Abstract: | Objective: To investigate the effects of paclitaxel on MMP-2,9 expression in ovarian carcinoma cells, and the possible underlying mechanism. Methods: The effect of paclitaxel on the proliferation of H08910 cell in vitro was determined by MTT(Thiazolyl blue) assay.The expressions of MMP-2,9 mRNA in H08910 cell were assayed by RT-PCR. Results: The IC50 of paclitaxel in H08910 cell were 6.22 x 10-7mol/L.The difference of growth inhibitory rate between 10-8mol/L paclitaxel and 10-7mol/L paclitaxel was significant(P<0.01) . Paclitaxel could down-regulate the expression of MMP-2,9 mRNA when the concentration was >10-8 mol/L. Conclusions: Ovarian carcinoma cell HO8910 is sensitive to paclitaxel when the concentration is >10-7 mol/L. Paclitaxel can inhibit the expression of MMP-2,9 mRNA. It may have some influence on invasion and metastasis of ovarian carcinoma. |
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| Keywords: | Metalloproteinases Paclitaxel Ovarian neoplasms Cell line H08910 Gene ex- pression |
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