氯化镉致癌机理的研究

应用氯化镉致人胚肺成纤维细胞恶性转化实验、染色体分析、流式细胞术对氯化镉的致癌机理进行研究。结果表明，氯化镉诱导的染色体畸变可能是其致癌机理之一；用流式细胞仪检测转化细胞突变型Ｐ５３蛋白和细胞周期素Ｄ１的表达，发现转化细胞中突变型Ｐ５３蛋白和细胞周期素Ｄ１的表达量均明显高于阴性对照组（Ｐ＜００１），初步证实了突变型Ｐ５３蛋白的表达及细胞周期素Ｄ１的过表达在镉致癌机理中起协同作用

Mechanism of Carcinogenesis Induced by CdCl 2 Department of Toxicology

The mechanism of carcinogenesis induced by CdCl 2 was studied through experiments of the malignant transformation of human embryo lung fibroblast in vitro,chromosome analysis and flow cytometry.on one hand,we think that it may be one of the carcinogenic mechanisms that CdCl 2 induces chromosomal aberration.On the other hand,the p 53 and cyclin D 1 expression quatities were determined by frow cytometry and the expressions of p 53 and cyclin D 1 in transformation cells were remarkably higher than control group(P<0 01),which preliminarily shows that p 53 protein cooperates with cyclin D 1 overexpression in playing a role during CdCl 2 induced carcinogenesis.this study will lay a foundation on further studying molecular mechanism of carcinogenesis induced by CdCl 2.