Antisense GAD67ODN对戊四氮所致慢性癫痫大鼠的作用

目的 :探讨谷氨酸脱羧酶 (GAD)的抗癫疒间作用。方法 :采用戊四氮制备大鼠慢性癫疒间 模型 ,利用反义寡脱氧核苷酸技术 ,通过选择性地抑制海马GAD基因的表达来观察其对慢性癫疒间大鼠行为、发作阈值及脑电图的影响。结果 :给予AntisenseGAD67ODN处理后 ,GADmRNA表达下降、GABA浓度下降、疒间 性发作潜伏期缩短、发作程度加重及脑电图疒间 波频率增加。结论 :从反面进一步说明GAD基因可能是抗疒间基因 ,为GAD基因治疗癫疒间提供实验依据

Effect of Antisense Glutamic Acid Decarboxylase (GAD) Oligodeoxynucleotide on the Epileptic Rats Induced by Pentylenetetrazol

Aim:To investigate effects of antisense glutamic acid decarboxylase (GAD) oligodeoxynucleotide (ODN) on behavior, seizure threshold and EEG of hippocampus in epileptic rats induced by pentylenetetrazol(PTZ).Methods:A model of chronic epilepsy in rats was established by PTZ. The inhibition of GAD mRNA expression in hippocampus was selectively induced by antisense oligodeoxynucleotide of GAD. The effect of antisense GAD ODN on behavior and EEG of hippocampus in epileptic rats was examined. Results:Antisense GAD ODN could inhibit the expression of GAD mRNA and the concentration of GABA. It could also significantly shorten the latencies of seizure and increase the level of seizure and the frequency of epileptiform discharges. Conclusion:The gene of GAD may be an anti seizure gene, which might inhibit epileptiform discharge. The treatment of epilepsy by GAD gene will have a bright future.